摘要
为发掘区分Fusarium asiaticum与Fusarium graminearum这两种赤霉病菌的特异性分子标记,从江淮麦区赤霉病穗上分离获得72个赤霉病菌株,进行种型与化学型鉴定,并利用SRAP引物对F.asiaticum-NIV、F.asiati-cum-3ADON及F.graminearum-15ADON 3种类型的致病菌进行种型及化学型特异性标记筛选。通过对特异性标记进行回收、转化与测序,根据序列设计出3个镰刀菌种型特异性SCAR引物,并用上述72个菌株进行验证。结果显示SCAR标记me1/em2-172仅在F.asiaticum中扩增出172 bp的特异性条带,标记me1/em6-311和me9/em4-639分别只在F.graminearum中扩增出311 bp和639 bp的特异性条带。表明,本研究获得的3个SCAR标记是F.asi-aticum和F.graminearum种型特异性标记,可用于区分这两种镰刀菌,为深入研究其致病机理及防治赤霉病的发生及危害提供了研究基础。
To develop specific markers for identifying the species and chemotypes of Fusarium asiaticum and Fusarium graminearum,infected spikes were collected from several wheat-growing regions between Yangtze River and Huaihe River,and seventy-two isolates collected from the diseased spikes were identified the genotype by primer pair Fg16F/Fg16R and chemotype by primer pair TOXP1/P2 and multiplex PCR.Sequence related amplified polymorphism（SRAP） markers were screened on representative isolates of F.asiaticum-NIV,F.asiaticum-3ADON and F.graminearum-15ADON to find species-specific and chemotype-specific products.Three species-specific SRAP markers were obtained by cloning and sequencing the specific products.Sequence-characterized amplified region（SCAR） primers specific for F.asiaticum and F.graminearum were designed based on the sequences of these species-specific products.The SCAR primers were tested with 72 isolates.The results showed that the primer pair me1/em2-172 could amplify a 172 bp PCR product only in F.asiaticum isolates,while the primer pairs of me1/em6-311 and me9/em4-639 could amplify 311 bp and 639 bp PCR products only in F.graminearum isolates,respectively.These results indicated that the SCAR primers designed in this study were species-specific primers for F.asiaticum and F.graminearum and could be used to identify these two types of Fusarium.The information obtained in this study provided a better understanding of the pathogenicity of Fusarium spp.and references for disease management.
出处
《江苏农业学报》
CSCD
北大核心
2012年第5期979-985,共7页
Jiangsu Journal of Agricultural Sciences
基金
国际科技合作项目(2009DFA32020)
现代农业产业技术体系项目(CARS-03)
