摘要
为探索小麦NBS-LRR类抗病基因的克隆方法、功能及作用机制,从小麦抗赤霉病品种宁7840中通过RT-PCR和RACE技术(末端快速扩增技术)得到一个新的NBS-LRR类抗病基因同源序列,命名为TaLRG,开放阅读框3 063 bp,编码1 021个氨基酸,具有NBS保守结构域和多个亮氨酸重复序列。聚类分析发现其编码蛋白质与水稻Pib在同一进化枝上。结构分析表明其编码蛋白质序列有4个疏水结构域,二级结构以卷曲和螺旋为主。用禾谷镰刀菌处理小麦赤霉病抗病和感病品种穗部后,利用RT-PCR进行半定量表达分析,发现该基因在病原菌侵染后上调表达显著,但在抗、感品种之间差别不显著。
A NBS-LRR gene was isolated from Triticum aestivum cv.Ning 7840 by RT-PCR and RACE-PCR techniques,named TaLRG.Sequence analysis showed the gene contained an open reading frame of 3 063 bp encoding a putative protein of 1 021 amino acids with NBS conservative structure domain and multiple leucine repeat sequences.Clustering analysis demonstrated that the deduced protein had high similarity with Pib from Oryza Satiua.Structure analysis proved that the protein sequence had four hydrophobic structure domains,and the secondary structure of the peptide was mainly composed of coils and helixes.It revealed an increased expression of TaLRG in the spikes of both Ning 7840（resistant） and Clark（susceptible） genotypes after infection by Fusarium graminearum.However,no significant difference was found in the expression between the two cultivars using semi-quantitative RT-PCR.
出处
《江苏农业学报》
CSCD
北大核心
2011年第6期1174-1180,共7页
Jiangsu Journal of Agricultural Sciences
基金
国家重大专项(2008ZX08002-001
2009ZX08002-011B)
科技部国际合作项目(2009DFA32020)
