摘要
【目的】钙依赖蛋白激酶(CDPK或CPK)广泛参与植物生长发育和环境胁迫响应。为探究CDPK基因在雨生红球藻(Haematococcus pluvialis)生长发育及虾青素积累中的功能,对HpCDPK基因家族进行鉴定和表达分析。【方法】利用生物信息学方法鉴定雨生红球藻HpCDPK基因家族成员,分析其编码蛋白的理化特性、系统进化、保守基序和功能结构域,以及缺氮等胁迫条件下HpCDPK成员基因的表达谱。【结果】结果表明,共鉴定7个HpCDPK基因家族成员,所有HpCDPK蛋白都含有典型EF-hand基序和蛋白激酶结构域。系统发育分析表明,这些HpCDPK与来自莱茵衣藻的CrCDPK聚为一类,与高等植物拟南芥的AtCDPK分开,暗示在进化过程中发生了种属特异性的基因复制事件。转录组数据分析表明,HpCDPK基因表达受到多种胁迫诱导,其中HpCDPK7基因转录水平在缺氮条件下上调最为明显。此外,HpCDPK与类胡萝卜素合成相关基因的表达相关性分析结果显示,HpCDPK与多个类胡萝卜素合成相关基因表达密切关联,特别是HpCDPK2与虾青素合成关键基因(BKT和BCH)的表达显著正相关(P<0.05)。【结论】本研究鉴定了7个HpCDPK基因,HpCDPK7基因在缺氮条件下的表达上调最为明显,HpCDPK2可能在类胡萝卜素及虾青素合成积累中发挥重要调控作用。研究结果为后续深入解析HpCDPK介导雨生红球藻胁迫响应和类胡萝卜素合成积累的功能及分子机制提供参考依据。
【Objective】Calcium-dependent protein kinases(CDPK or CPK)are widely involved in plant growth and development and environmental stress response.To explore their roles involved in Haematococcus pluvialis growth,development and astaxanthin accumulation,HpCDPK gene family was identified and analyzed.【Method】Bioinformatics tools were used to identify the HpCDPK gene family members,and to analyze the physical and chemical properties,phylogenetic evolution,conserved motifs and functional domains of the encoded proteins,as well as the gene expression profiles under nitrogen deficiency stress.【Result】The results showed that seven members of HpCDPK gene family were identified,and all HpCDPK proteins contained typical EF-hand motifs and protein kinase domains.Phylogenetic analysis showed that these HpCDPKs clustered with the CrCDPKs from Chlamydomonas reinhartii and separated from the AtCDPKs of Arabidopsis thaliana,suggesting that species-specific gene replication occurred in the process of evolution.Transcriptome data analysis showed that HpCDPK gene expression was induced by a variety of stresses,among which the transcription level of HpCDPK7 gene was most significantly up-regulated under nitrogen deficiency.In addition,the analysis of the correlation between HpCDPK and the expression of genes related to carotenoid synthesis showed that HpCDPK was closely related to the expressions of genes relevant to several carotenoid synthesis in H.pluvialis,especially HpCDPK2 was positively correlated with the expression of key genes of astaxanthin synthesis(BKT and BCH).【Conclusion】Seven HpCDPK genes were identified,the expression of HpCDPK7 gene was most significantly up-regulated under nitrogen deficiency and HpCDPK2 may play an active role in the synthesis and accumulation of carotenoids and astaxanthin.The results provide a reference basis for further analysis of the function and molecular mechanism of stress response and carotenoids synthesis and accumulation mediated by HpCDPK in H.pluvialis.
作者
李亚男
张豪杰
梁梦静
罗涛
李旺宁
张春辉
季春丽
李润植
薛金爱
崔红利
LI Ya-nan;ZHANG Hao-jie;LIANG Meng-jing;LUO Tao;LI Wang-ning;ZHANG Chun-hui;JI Chun-li;LI Run-zhi;XUE Jin-ai;CUI Hong-li(College of Agriculture,Shanxi Agricultural University,Institute of Molecular Agriculture and Bioenergy,Taigu 030801;Yantai Institute of Coastal Zone Research,Chinese Academy of Sciences,Key Laboratory of Coastal Biology and Bioresource Utilization,Yantai 264003)
出处
《生物技术通报》
CAS
CSCD
北大核心
2024年第2期300-312,共13页
Biotechnology Bulletin
基金
国家重点研发计划项目(2021YFD1901105)
国家自然科学基金项目(31902394和41876188)
山西省高等学校科技创新计划(2021L119)
山西农业大学科技创新基金(2018YJ16)。
关键词
雨生红球藻
钙依赖性蛋白激酶
序列分析
表达模式
非生物逆境
Haematococcus pluvialis
calcium-dependent protein kinase
sequence analysis
expression pattern
abiotic stress
