摘要
干旱严重影响玉米的产量和品质。钙依赖性蛋白激酶(Calcium-dependent protein kinase,CPK)是一种Ca^(2+)结合蛋白,在植物非生物胁迫应答方面发挥重要作用。本研究对玉米37个ZmCPKs的染色体定位、系统进化、基因结构、保守结构域和理化性质进行了分析。其中,36个ZmCPK基因不均匀的分布在9条染色体上;通过系统进化分析将其分为4个亚组,同一亚组成员具有相似的基因结构和保守结构域;ZmCPKs启动子分析表明,ZmCPKs启动子区含有大量的逆境响应元件,包括MBS、DRE和ABRE等;qTeller数据库和转录组数据分析表明,ZmCPKs基因家族成员在营养生长和生殖生长期均有表达,其中,26个基因在干旱胁迫下表达量发生变化。综合启动子元件及转录组数据,共筛选出9个基因作为干旱响应候选基因。进一步qRT-PCR验证显示,这9个基因均受PEG诱导表达。本研究可为玉米耐旱性的遗传改良提供优异的基因资源。
Maize is one of the major important food crops in China.Drought seriously affects the yield and quality of maize.The calcium-dependent protein kinase(CPK),a Ca^(2+)binding protein,plays an important role in plant growth and development,as well as abiotic stress response.In this study,the chromosome localization,phylogeny,gene structure,conserved domains and physicochemical properties of 37 ZmCPKs were analyzed.Among them,36 ZmCPK genes were unevenly distributed on 9 chromosomes.Through the phylogenetic analysis,the CPKs were divided into four subgroups,and members of the same subgroup demonstrated similar structures and conserved domains.Analysis of ZmCPK promoters showed that the ZmCPKs promoter regions contained a large number of stress responsive elements,including MBS,DRE and ABRE.The expression analysis with the qTeller database and transcriptome data showed that ZmCPK genes were expressed in the vegetative and reproductive growth stages,and 26 ZmCPK genes responded to drought stress.According to the results of cis-elements prediction and transcription analysis,9 genes were selected as candidate genes responding to drought,which was further confirmed by qRT-PCR analysis showing induction of their expression by PEG treatment.This study provides resources for the genetic improvement of maize drought tolerance.
作者
邢悦
杨倩
钟源
翟秀珍
王楠
陶勇生
段会军
XING Yue;YANG Qian;ZHONG Yuan;ZHAI Xiuzhen;WANG Nan;TAO Yongsheng;DUAN Huijun(College of Agronomy/State Key Laboratory of North China Crop Improvement and Regulation/North China Key Laboratory for Crop Germplasm Resources of Education Ministry,Hebei Agricultural University,Baoding 071001,China)
出处
《河北农业大学学报》
CAS
CSCD
北大核心
2023年第5期8-17,共10页
Journal of Hebei Agricultural University
基金
河北省重点研发计划项目(21326328D)
河北省现代农业产业技术体系玉米产业创新团队建设项目(HBCT2018020207)
河北省省属高校基本科研业务费研究项目(KY2021038).
