摘要
本研究旨在探明加味小柴胡汤结合捏脊法治疗心肌炎(MC)的作用机制。方法:将30只小鼠随机分成空白组、模型组、西药组、柴治组、捏治组、合治组共6组,每组5只。空白组不造模,其余各组均制成自身免疫性MC模型。各组施以相应处理7 d,结束后取心肌组织和血清分别检测肿瘤坏死因子-ɑ(TNF-ɑ)、白细胞介素-1β(IL-1β)、肌酸激酶同工酶(CK-MB)、心肌肌钙蛋白T(cTnT)、Toll样受体4(TLR4)、核因子-κB p65(NF-κB p65)、B淋巴细胞瘤-2基因(Bcl-2)、Bcl-2相关X蛋白(Bax)、半胱氨酸天冬氨酸蛋白酶-3(Caspase-3)的含量及心肌切片观察。结果:中医各治疗组对阻断心肌TLR4/NF-κB p65炎症通路及抑制心肌细胞凋亡的作用均优于西药组(P<0.05),且合治组在降低NF-κB p65、TNF-ɑ、Bax、Caspase-3和升高Bcl-2中的作用较其余治疗组为优(P<0.05);同时,与西药组比较,各中医治疗组心肌切片显示心肌细胞排列紊乱均有不同程度改善,心肌间隙炎细胞浸润较少,以合治组表现最佳。结论:加味小柴胡汤结合捏脊法可能通过抑制心肌TLR4/核因子-κB(NF-κB)信号通路阻止心肌炎症反应,同时抑制凋亡因子减少心肌凋亡来发挥对MC的治疗作用;二者结合运用能发挥良好的协同作用。
This study aims to explore the mechanism of modified Xiaochaihu Decoction combined with spine pinching in the treatment of myocarditis(MC).Thirty mice were randomized into blank,model,Western medicine,modified Xiaochaihu Decoction,spine pinching,and combined treatment(modified Xiaochaihu Decoction+spine pinching)groups,with 5 mice in each group.The mice in other groups except the blank group were modeled for autoimmune MC.After each group was treated for 7 days,the myocardium tissue and serum were collected,and the levels of tumor necrosis factoralpha(TNF-α),interleukin-1 beta(IL-1β),creatine kinase-MB(CK-MB),cardiac troponin(cTnT),Tolllike receptor 4(TLR4),nuclear factor-kappa B p65(NF-κ-p65),B-cell lymphoma-2(Bcl-2),Bcl-2-associated protein X(Bax),and cysteinyl aspartate-specific protease(caspase-3)were measured.The myocardium sections were observed.The results showed that the Chinese medical therapies outperformed Western medicine in blocking the TLR4/NF-κF p65 pathway and inhibiting apoptosis in the myocardium(P<0.05).The combined treatment group outperformed other groups in lowering the levels of NF-κB p65,TNF-α,Bax,and caspase-3 and elevating the level of Bcl-2(P<0.05).Compared with the Western medicine group,Chinese medical therapies repaired the myocardial cell disarrangement and reduced the infiltration of inflammatory cells in the myocardium,and the combined treatment showed the best performance.In conclusion,modified Xiaochaihu Decoction combined with spine pinching can prevent myocardial inflammation and reduce apoptosis by inhibiting the TLR4/NF-κB signaling pathway.The combination can play a synergistic role in the treatment of MC.
作者
秦宇航
黄丽丽
王建珠
杨小存
张淑敏
QIN Yuhang;HUANG Lili;WANG Jianzhu;YANG Xiaocun;ZHANG Shumin(School of Acupuncture-Moxibustion and Tuina/School of Health Preservation and Rehabilitation,Nanjing University of Chinese medicine,Nanjing 210023,China;Contraceptives Adverse Reaction Surveillance Center,National Health Commission,Jiangsu Health Development Research Center,Nanjing 210036,China)
出处
《基层中医药》
2024年第1期33-38,共6页
Basic Traditional Chinese Medicine
关键词
脏腑别通
加味小柴胡汤
捏脊法
心肌炎
心-胆-胃轴
zang-fu extraordinary connection
modified Xiaochaihu Decoction
spine pinching
myocarditis
heart-gallbladder-stomach axis
