摘要
目的探讨微小RNA(miR)-27a和miR-128在种植体周围炎患者龈沟液中的水平及其与骨吸收的关系。方法将2018年1月至2021年12月于上海长征医院口腔科完成牙种植修复术的患者136例纳入研究,根据种植牙状况分为健康种植体患者66例(健康组)和种植体周围炎患者70例(炎症组)。另外,选取同期进行牙龈健康检查的志愿者70例作为对照组。收集所有纳入研究者探诊深度(PD)、龈沟出血指数(SBI)、龈沟液量、肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-6、IL-4水平的数据。采用口腔锥形束CT(CBCT)进行骨吸收测定。采用实时荧光定量聚合酶链反应(qPCR)检测龈沟液中miR-27a和miR-128水平。采用Pearson相关法分析种植体周围炎患者龈沟液中miR-27a和miR-128水平及二者与炎症因子、骨吸收量的相关性。结果与对照组相比,炎症组和健康组龈沟液中PD、SBI、龈沟液量、骨吸收量,TNF-α、IL-6、miR-128水平升高,IL-4、miR-27a水平降低,差异均有统计学意义(P<0.05);与健康组相比,炎症组龈沟液中PD、SBI、龈沟液量、骨吸收量、TNF-α、IL-6、miR-128水平升高,IL-4、miR-27a水平降低,差异均有统计学意义(P<0.05)。相关性分析显示,种植体周围炎患者龈沟液中miR-27a和miR-128水平呈负相关(r=-0.365,P=0.002)。种植体周围炎患者龈沟液中miR-27a水平与PD、SBI、骨吸收量、TNF-α、IL-6水平呈负相关(P<0.05),与IL-4水平呈正相关(P<0.05);miR-128水平与PD、SBI、骨吸收量、TNF-α、IL-6水平呈正相关(P<0.05),与IL-4水平呈负相关(P<0.05)。结论miR-128在种植体周围炎患者龈沟液中呈高水平,miR-27a呈低水平,二者与骨吸收相关。
Objective To investigate the levels of miR-27a and miR-128 in gingival crevicular fluid(GCF)of patients with peri-implantitis and their relationship with bone resorption.Methods A total of 136 patients who completed dental implant restoration in the Department of Stomatology,Shanghai Changzheng Hospital from January 2018 to December 2021 were enrolled in the study.According to the status of dental implants,they were divided into 66 healthy implant patients(healthy group)and 70 peri-implantitis patients(inflammation group).In addition,70 volunteers who underwent gingival health examination during the same period were enrolled as the control group.The data of probing depth(PD),sulcus bleeding index(SBI),volume of gingival crevicular fluid,and levels of tumor necrosis factor-α(TNF-α),interleukin(IL)-6,and IL-4 were collected.Bone resorption was measured by oral cone beam computed tomography(CBCT).The levels of miR-27a and miR-128 in gingival crevicular fluid were detected by fluorescence quantitative polymerase chain reaction(qPCR).Pearson correlation analysis was used to analyze the levels of miR-27a and miR-128 in gingival crevicular fluid of patients with peri-implantitis and their correlation with inflammatory factors and bone resorption.Results Compared with the control group,PD,SBI,GCF volume,bone resorption,and the levels of TNF-α,IL-6,and miR-128 in the inflammation group and the healthy group increased,while the levels of IL-4 and miR-27a decreased,and the differences were statistically significant(P<0.05).Compared with the healthy group,PD,SBI,GCF volume,bone resorption,TNF-α,IL-6,and miR-128 levels in the inflammation group significantly increased,and IL-4 and miR-27a levels significantly decreased(P<0.05).Correlation analysis showed that the levels of miR-27a and miR-128 in gingival crevicular fluid of patients with peri-implantitis were negatively correlated(r=-0.365,P=0.002).The level of miR-27a in gingival crevicular fluid of patients with peri-implantitis was negatively correlated with PD,SBI,bon
作者
沈苗苗
陈玲
翟云霞
SHEN Miaomiao;CHEN Lin;ZHAI Yunxia(Department of Stomatology,Shanghai Changzheng Hospital,Shanghai 200001,China;Department of Pathology,Longhua Hospital Affiliated to Shanghai University,Shanghai 230032,China)
出处
《国际检验医学杂志》
CAS
2024年第5期513-516,522,共5页
International Journal of Laboratory Medicine
基金
上海市科学技术计划项目(174119678)。
