摘要
目的建立HPLC-UV法测定金钱草中紫云英苷、山柰酚-3-O-芸香糖苷、槲皮素、山柰酚和异鼠李素。方法采用Eclipse XDB-C18色谱柱(150 mm×4.6 mm,3.5μm),流动相为甲醇–0.4%磷酸水,梯度洗脱,检测波长360 nm,体积流量0.5 mL/min,柱温30℃,进样量10μL。结果紫云英苷、山柰酚-3-O-芸香糖苷、槲皮素、山柰酚、异鼠李素分别在22.10~176.80、21.14~169.12、17.40~174.00、51.12~408.96、23.30~186.40μg/mL呈现良好线性关系(R^(2)>0.9991);平均加样回收率分别为99.50%、100.39%、99.93%、105.58%、98.96%,RSD值分别为1.07%、1.44%、0.57%、0.24%、0.65%(n=6)。结论方法的重复性好,操作简单,结果准确,可以用来控制金钱草药材及其制剂的质量。
Objective To develop an HPLC method for determination of astragalin,kaemperfol-3-O-rutinoside,quercetin,kaempferol,and isorhamnetin in Lysimachia christinae.Methods The analysis was performed on Eclipse XDB-C18 column(150 mm×4.6 mm,3.5μm).The mobile phase consisted of methanol and 0.4%phosphoric acid with gradient elution.The detection wavelength was 360 nm,flow rate was 0.5 mL/min,column temperature was 30℃,and volume of injection was 10μL.Results Aastragalin,kaemperfol-3-O-rutinoside,quercetin,kaempferol,and isorhamnetin showed good linear relationships of 22.10—176.80,21.14—169.12,17.40—174.00,51.12—408.96,and 23.30—186.40μg/mL(R^(2)≥0.9991),whose average recoveries were 99.50%,100.39%,99.93%,105.58%,and 98.96%with RSD values of 1.07%,1.44%,0.57%,0.24%,and 0.65%(n=6),respectively.Conclusion The method is reproducible,simple,and accurate,which can be used for the quality control of Lysimachia christinae and its preparations.
作者
吴凡
黄昌晶
李洁
程丹
张伟
徐步斌
周斌
WU Fan;HUANG Changjing;LI Jie;CHENG Dan;ZHANG Wei;XU Bubin;ZHOU Bin(School of Pharmacy,Jiangxi Science and Technology Normal University,Nanchang 330013,China)
出处
《现代药物与临床》
CAS
2024年第1期102-105,共4页
Drugs & Clinic
基金
国家自然科学基金资助项目(81760701)。
