摘要
蓝莓果渣中的花色苷经超声辅助乙醇法进行提取,通过正交试验确定提取的最优条件为料液比1∶30(g/mL)、pH 3.0、乙醇体积分数50%、超声时间30 min,然后利用大孔树脂对粗提得到的花色苷进行分离纯化,对比了XDA-6、LX-16、LX-41、LX-180S、LK825、DM216种大孔树脂的吸附和解吸效果。结果表明,LK825型大孔树脂的吸附率和解吸率分别为90.36%、90.19%,具有较强的吸附能力和较好的解吸性能,故选用LK825型大孔树脂进行纯化试验。LK825型大孔树脂静态试验的最优条件为吸附平衡时间7 h、解吸平衡时间5 h、提取液pH 3.0、解吸液pH 3.0、解吸液乙醇体积分数60%。动态试验的最优参数为上样流速1.0 mL/min、上样液体积22 BV、上样浓度1.0 mg/mL、洗脱流速1.5 mL/min、解吸液体积9 BV。纯化后蓝莓果渣花色苷的含量和色价分别是纯化前的12.8倍和17.9倍,纯度明显提高。
The anthocyanins in blueberry residue are extracted by ultrasonic-assisted ethanol method.The optimal extraction conditions determined through orthogonal experiment are solid-liquid ratio of 1∶30(g/mL),pH of 3.0,ethanol volume fraction of 50%and ultrasonic time of 30 min,and then macroporous resins are used to separate and purify the anthocyanins obtained by crude extraction.The adsorption and desorption effects of six macroporous resins such as XDA-6,LX-16,LX-41,LX-180S,LK825,DM21 are compared.The results show that the adsorption rate and desorption rate of LK825 macroporous resin are 90.36% and 90.19% respectively,which has strong adsorption capacity and good desorption property.Therefore,LK825 macroporous resin is selected for purification test.The optimal conditions for the static test of LK825 macroporous resin are adsorption equilibrium time of 7 h,desorption equilibrium time of 5 h,extraction solution pH of 3.0,desorption solution pH of 3.0 and ethanol volume fraction in desorption solution of 60%.The optimal parameters of dynamic test are loading flow rate of 1.0 mL/min,loading solution volume of 22 BV,loading concentration of 1.0 mg/mL,elution flow rate of 1.5 mL/min and desorption solution volume of 9 BV.After purification,the content and color value of anthocyanins in blueberry residue are 12.8 times and 17.9 times of those before purification,and the purity is significantly improved.
作者
张芷瑜
张天伟
杨馥菡
李会珍
张志军
ZHANG Zhi-yu;ZHANG Tian-wei;YANG Fu-han;LI Hui-zhen;ZHANG Zhi-jun(College of Chemistry and Chemical Engineering,North University of China,Taiyuan 030051,China;Jinzhong Industrial Technology Innovation Institute,North University of China,Jinzhong 030600,China)
出处
《中国调味品》
CAS
北大核心
2024年第3期175-181,共7页
China Condiment
基金
山西省重点研发计划项目特优农业技术创新专项(2022ZDYF123)。
关键词
蓝莓果渣
花色苷
提取
纯化
blueberry residue
anthocyanins
extraction
purification
