摘要
为了除去紫甘薯提取液中的糖类杂质,采用乙醇/硫酸铵双水相体系纯化紫甘薯色素提取液。通过设计ω(乙醇)、ω(硫酸铵)、色素提取液pH以及ω(色素提取液)四个因素的单因素实验和正交实验来探究和优化实验条件。通过正交实验,以选择性系数为考察指标,确定了各因素对紫甘薯色素纯化的影响程度为:ω(乙醇)>ω(硫酸铵)>pH>ω(色素提取液);最优纯化条件是:ω(乙醇)为25.0%,ω(硫酸铵)为17.0%,色素提取液pH为3.5,ω(色素提取液)为11.0%,此时的选择性系数α为242.07,色素保留率为98.88%,糖类去除率为85.25%。该方法分离纯化紫甘薯色素产品易回收、生产成本低,可提高企业的经济效益,有望后续进行工业化生产。
In order to remove the saccharide impurities in purple sweet potato extract,aqueous two-phase system of ethanol-ammonium sulfate is used to purify the extract.The single factor experiments and orthogonal experiments based on ethanol,ammonium sulfate,pigment extract and pH,are investigated to explore and optimize the process conditions.The influence degree of each factor on the purification of purple sweet potato pigment are determined by the orthogonal experiment with the selectivity coefficient(α)as an index:ethanol mass fraction>ammonium sulfate mass fraction>pH>pigment extract addition amount.The optimum parameters are as follows:ethanol 25.0%,ammonium sulfate 17%,pH 3.5,and pigment extract 11.0%,resulting in a selectivity coefficient of 242.07,a pigment retention rate of 98.88%and a sugar removal rate of 85.25%.The results indicate that this method can effectively separate and purify purple sweet potato pigment with easy recovery of product and low production cost,which can improve the economic benefits of enterprise,and is promising for subsequent industrial production.
作者
王云颖
尚彦文
高灿
秦海宁
刘国凯
展康华
常秀莲
WANG Yun-ying;SHANG Yan-wen;GAO Can;QIN Hai-ning;LIU Guo-kai;ZHAN Kang-hua;CHANG Xiu-lian(School of Life Sciences,Yantai University,Yantai 264005,China;Qingdao Pengyuan Kanghua Natural Products Co Ltd,Qingdao 266612,China)
出处
《烟台大学学报（自然科学与工程版）》
CAS
2023年第2期248-252,共5页
Journal of Yantai University(Natural Science and Engineering Edition)
基金
横向课题(SK18H08,SK19H21)。
关键词
紫甘薯
花青素
双水相萃取
Solanum tuberdsm
anthocyanin
aqueous two-phase system
