摘要
目的:建立奇白消软膏的定性鉴别与含量测定方法,加强奇白消软膏的质量控制。方法:采用薄层色谱(TLC)法对制剂中的黄连和人工牛黄进行定性鉴别;采用高效液相色谱法(HPLC)测定制剂中黄连有效成分盐酸小檗碱的含量。结果:奇白消软膏的TLC斑点清晰、阴性无干扰、分离度好;盐酸小檗碱在1.28~38.4μg;回归方程为Y=49873.84X-554.74(r=1)。精密度、稳定性、重复性试验的RSD分别为0.41%、0.38%、1.8%;加样回收率为98.88%~101.23%,RSD为0.95%(n=6)。结论:本研究建立的TLC和HPLC简便易行,准确、重复性好,可用于奇白消软膏的质量控制。
Objective To establish the identification and content determination methods of Qibaixiao Ointment,to enhance the quality controllability of Qibaixiao Ointment.Methods Thin layer chromatography method(TLC)was used for qualitative identification of Coptis chinensis Franch,Bovis calculus Artifactus.Using high performance liquid chromatography(HPLC)was used for content determination of Berberine hydrochloride.Results The TLC spots were clear spots with less negative interference,well separated and good reproducibility.Berberine hydrochloride showed a good linear relationship in the range of 1.28-38.4μg.The regression equation was:Y=49873.84X-554.74(r=1).The RSD values of precision,repeatability and stability experiments were 0.41%,0.38%,1.8%.The average recovery rates was 98.88%-101.23%with RSD was 0.95%(n=6).Conclusion The qualitative identification method and content determination method established method in this study for Qibaixiao Ointment are easy to operate with good reproducibility and accurate,reliable.It can be used to evaluate the quality of Qibaixiao Ointment.
作者
梁林辉
章斌
姚永秀
彭东
刘德军
赵君
LIANG Linhui;ZHANG Bin;YAO Yongxiu;PENG Dong;LIU Dejun;ZHAO Jun(School of Pharmacy and Medical Laboratory Science,Ya'an Polytechnic College,Ya'an,625000,China;Ya'an Food and Drug Application Development Research Center,Ya'an,625000,China;Ya'an Puyuan Biotechnology Co.,Ltd,Ya'an,625000,China;School of pharmacy,Chengdu Medical College,Chengdu,610000,China)
出处
《中国民族民间医药》
2024年第1期28-32,共5页
Chinese Journal of Ethnomedicine and Ethnopharmacy
基金
四川省中医药管理局项目:2020CP0031
雅安市2021年度省级转移支付项目:22ZYZF0003。
