摘要
目的研究冰片对长时连续作业大鼠前额叶皮层单胺类递质水平的影响。方法采取跑台训练方法,建立大鼠长时连续作业模型。连续作业结束后,灌胃给予冰片(0.4 g/kg),测试其对大鼠随后夜间行为状态分布模式及主动回避行为的影响。采用高效液相-电化学方法测定大鼠前额叶皮层单胺类神经递质水平的变化。结果跑台冰片组夜间活动明显高于跑台石蜡组;跑台冰片组主动回避反应时间(6.26±0.96)s较跑台石蜡组(6.85±1.24)s显著降低。跑台石蜡组前额叶皮层单胺类递质包括去甲肾上腺素、5-羟色胺和多巴胺水平较对照组下降,而给予冰片后去甲肾上腺素和5-羟色胺水平显著增加。结论冰片可明显改善长时连续作业大鼠的觉醒水平,这种作用与其增加前额叶皮层去甲肾上腺素和5-羟色胺水平有关。
Objective To investigate the effect of borneol on levels of monoamine neurotransmitters in the prefrontal cortex of rat after long-term continuous operations. Methods A rat model of long-term continuous operations were established by using a treadmill protocol. Then Borneol （0.4 g/kg, p. o. ） was given to the rats after the operation, and their activity-rest behavior and active avoidance response were assessed. Two h later, the rats were sacrificed and the level of monoamine neurotransmitter in the prefrontal cortex was determined by high performance liquid chromatography combined with electrochemical detection （HPLC-ECD）. Results After the administration of borneol, the activities of operational animals were increased markedly as compared with that of vehicle operational group. The operational rats after borneol application had less response time [ （6.26 ＋0.96） s ] than the ones treated with vehicle （6.85 ± 1.24） s in active avoidance response. The levels of monoamine neurotransmitters including noradrenaline, dopamine, and 5-serotonin in the prefrontal cortex were decreased after treadmill operation. While application of borneol made the levels of noradrenaline and 5-serotonin increased significantly as compared to operational group. Conclusion Borneol have a reversal effect on rat awake activity impairment after long-term continuous operations. And the arousal effects of borneol are associated with the increase of noradrenaline and 5-serotonin levels in the prefrontal cortex.
出处
《第三军医大学学报》
CAS
CSCD
北大核心
2006年第18期1867-1869,共3页
Journal of Third Military Medical University
基金
第三军医大学军事医学创新科研基金资助项目(XJYY200310)
全军"十一五"面上项目(06MA195)~~
关键词
长时连续作业
觉醒
冰片
大鼠
long-term continuous operations
wakefulness
borneol
rat
