摘要
为了解河南省2021-2022年犊牛腹泻病原流行情况,利用犊牛腹泻病原微生物核酸检测试剂盒(荧光PCR法)分别对2021年河南省20余家牧场送检的170份犊牛腹泻样品,2022年送检的112份犊牛腹泻样品进行牛轮状病毒(BRV)、牛冠状病毒(BCoV)及大肠杆菌K99^(+)(E.coli K99^(+))荧光PCR检测,并对检测结果采用SPSS 27.0软件进行数据统计以及显著性差异分析(Pearson卡方检验)。结果显示:2022年BRV检出率为50.00%、BCoV检出率为41.00%,相比2021年均显著增高(P<0.01),E.coli K99^(+)检出率与上一年相比基本持平,无明显差异(P>0.05),说明2022年BRV、BCoV流行情况愈加严重。进一步分析发现,2022年腹泻病原单一感染检出率为26.79%,其中主要是BRV感染,与2021年单一感染检出率25.29%基本持平,差异不显著(P>0.05)。2022年腹泻病原混合感染检出率为35.71%,且主要以BRV、BCoV混合感染为主。相比2021年的12.94%明显上升,差异显著(P<0.01),说明2022年混合感染情况愈加严重。建议针对河南省腹泻流行情况有针对性地开展防控与净化。本检测初步摸清了2021年、2022年河南省不同地区犊牛腹泻主要病原流行情况,可为河南省制定犊牛腹泻病综合防控措施提供数据支撑。
In order to make sure the prevalence of calf diarrhea pathogens in Henan Province from 2021 to 2022,170 calf diarrhea samples from more than 20 pastures in Henan Province were tested by using a nucleic acid detection+kit(fluorescent PCR).Bovine rotavirus(BRV),bovine coronavirus(BCoV)and bovine Escherichia coli K99^(+)(E.coli K99^(+))were detected by PCR in 112 samples of calf diarrhea tested in 2022.and the results were summarized by SPSS 27.0 software and analyzed by Pearson's chi-squared test.It was shown that:In 2022,the detection rate of BRV and BCoV pathogens was 50.00%and 41.00%,significantly higher than that of 2021(P<0.01).The detection+rate of E.coli K99^(+) was basically unchanged compared with that of the previous year(P>0.05),indicating that the prevalence of BRV and BCoV was more and more serious in 2022.Further analysis showed that the detection rate of diarrhea pathogen single infection in 2022 was 26.79%,mainly BRV virus infection,which was basically the same as that of 2021,which was 25.29%,and the difference was not significant(P>0.05).In 2022,the detection rate of diarrhea mixed infection was 35.71%,and BRV and BCoV mixed infection was the main infection.Compared with 12.94%in 2021,the difference was significant(P<0.01),indicating that the mixed infection was more serious in 2022,so it is suggested to carry out targeted prevention and control and purification of diarrhea epidemic in Henan Province.This test preliminarily identified the infection situation of the three pathogens of calf diarrhea in different regions of Henan Province in 2021 and 2022,which can provide certain data support for the formulation of comprehensive prevention and control measures of calf diarrhea in Henan Province.
作者
王向佩
周峰
薛永康
秦帅
张洋
闫磊
闫跃飞
刘晓曼
孙贝贝
谷淑华
张震
WANG Xiang-pei;ZHOU Feng;XUE Yong-kang;QIN Shuai;ZHANG Yang;YAN Lei;YAN Yue-fei;LIU Xiao-man;SUN Bei-bei;GU Shu-hua;ZHANG Zhen(Henan Dairy Cow production Performance Measurement Co.,LTD.,Zhengzhou 450000;Henan Seed Industry Development Center,Zhengzhou 450000;Henan Breeding and Poultry Industry Research Institute Co.,Ltd.Zhengzhou 450046;Henan Dairy Cow production Performance Testing Center,Zhengzhou 450000;Henan International Joint Laboratory of Dairy Cattle Health,Zhengzhou 450000)
出处
《中国奶牛》
2024年第2期25-30,共6页
China Dairy Cattle
基金
河南省现代农业产业技术体系建设专项资金资助(HARS-22-14-S)
河南省重点研发专项(221111111100)
河南省科技攻关(222102520042)。
