摘要
乳酸菌因其可以增强免疫力、促进消化、降低血清胆固醇等多种生理功能而受到了广泛关注。为进一步提升发酵乳酸菌的增殖密度,本实验以植物乳杆菌AR307为试验对象,MRS培养基为基础培养基,采用单因素及响应面试验设计对培养基进行优化。研究碳源、氮源、无机盐对菌株生长的影响,以OD_(600)为评价指标确定最佳配比。结果表明:最优碳源为麦芽糖,添加量为3.5%;最优氮源为酵母浸粉、酪蛋白胨与牛肉浸出粉,比例为1∶1∶1,添加量为2.21%;最优无机盐为磷酸氢二钾,添加量为0.75%。在此优化条件下,静置发酵24 h,植物乳杆菌AR307的OD_(600)值达到了6.4,菌体密度得到了提升,为AR307工业化发酵提供了理论依据。
Lactic acid bacteria have received widespread attention and research due to their various physiological functions such as enhancing immunity,promoting digestion,and reducing serum cholesterol.To further enhance the proliferation density of fermented lactic acid bacteria,this experiment used Lactobacillus plantarum AR307 as the experimental object,MRS medium as the basic medium,and optimized the medium using single factor and response surface methodology.The paper studies the effects of carbon sources,nitrogen sources,and inorganic salts on bacterial growth,and determines the optimal ratio using OD600 as the evaluation index.The results show that the optimal carbon source is maltose,with an added amount of 3.5%;The optimal nitrogen source is yeast extract powder,casein peptone,and beef extract powder,with a ratio of 1∶1∶1 and an added amount of 2.21%;The optimal inorganic salt is potassium dihydrogen phosphate,with a dosage of 0.75%.Under these optimized conditions,after 24 h of fermentation,the OD600 value of Lactobacillus plantarum AR307 reached 6.4,and the bacterial density was improved,providing a theoretical basis for the industrial fermentation of AR307.
作者
衡利冰
夏永军
艾连中
刘欣欣
HENG Libing;XIA Yongjun;AI Lianzhong;LIU Xinxin*(School of Healthy Science and Engineering,Shanghai Engineering Research Center of Food Microbiology,University of Shanghai for Science and Technology,Shanghai 200093,China)
出处
《工业微生物》
CAS
2024年第1期81-86,共6页
Industrial Microbiology
基金
上海市教育委员会科研创新计划(2101070007800120)
国家自然科学基金(32101922)。
关键词
植物乳杆菌
培养基优化
响应面
Lactobacillus plantarum
media optimization
response surface method
