摘要
利用酵母提取物(yeast extract, YE)作为模拟生物胁迫的诱导子,诱导花楸悬浮细胞(Sorbus pohuashanensis suspension cell, SPSC)产生生物胁迫作用,对胁迫下产生的三萜类次生代谢产物进行分离鉴定。采用正相硅胶、葡聚糖凝胶、中压制备液相等色谱技术对SPSC甲醇提取物进行分离纯化,并运用波谱分析技术鉴定出26个三萜类化合物,包括15个乌苏烷三萜(1~15),2个18,19-裂环乌苏烷三萜(16~17),4个羽扇豆烷三萜(18~21),2个环阿屯烷三萜(22~23),以及3个鲨烯型三萜(24~26)。所有三萜均为首次从YE诱导SPSC中分离得到,其中化合物22-O-acetyltripterygic acid A(1)为新化合物。对分离得到的化合物进行抗真菌、抗肿瘤和抗炎活性评估,结果表明化合物1对脂多糖(LPS)诱导的小鼠巨噬细胞RAW264.7释放一氧化氮(NO)具有一定的抑制作用。
This study induced biological stress in Sorbus pohuashanensis suspension cell(SPSC) with yeast extract(YE) as a bio-tic elicitor and isolated and identified secondary metabolites of triterpenoids produced under stress conditions. Twenty-six triterpenoids, including fifteen ursane-type triterpenoids(1-15), two 18,19-seco-ursane-type triterpenoids(16-17), four lupine-type triterpenoids(18-21), two cycloartane-type triterpenoids(22-23), and three squalene-type triterpenoids(24-26), were isolated and purified from the methanol extract of SPSC by chromatography on silica gel, MCI, Sephadex LH-20, and MPLC. Their structures were elucidated by spectroscopic analyses. All triterpenoids were isolated from SPSC for the first time and 22-O-acetyltripterygic acid A(1) was identified as a new compound. Selected compounds were evaluated for antifungal, antitumor, and anti-inflammatory activities, and compound 1 showed an inhibitory effect on NO production in LPS-induced RAW264.7 cells.
作者
张琴
李强
王健
蒋汶利
海萍
贾春梅
任柳怡
吴旭东
康传志
杨健
高原
郭兰萍
ZHANG Qin;LI Qiang;WANG Jian;JIANG Wen-li;HAI Ping;JIA Chun-mei;REN Liu-yi;WU Xu-dong;KANG Chuan-zhi;YANG Jian;GAO Yuan;CUO Lan-ping(Faculty of Materials and Chemical Engineering,Yibin University,Yibin 644000,China;State Key Laboratory for Quality Ensurance and Sustainable Use of Dao-di Herbs,National Resource Center for Chinese Materia Medica,China Academy of Chinese Medical Sciences,Beijing 100700,China)
出处
《中国中药杂志》
CAS
CSCD
北大核心
2024年第1期130-140,共11页
China Journal of Chinese Materia Medica
基金
国家自然科学基金重大项目(81891014)
国家自然科学基金青年基金项目(21702181)
中国中医科学院科技创新工程项目(CI2021A03903,CI2021A03905,CI2021B013)
国家中医药管理局中医药创新团队及人才支持计划项目(ZYYCXTD-D-202005)
财政部和农业农村部国家现代农业产业技术体系项目(CARS-21)
宜宾学院校级培育项目(412-2020PY05)。
关键词
花楸悬浮细胞
生物胁迫
化学成分
三萜
抗炎活性
Sorbus pohuashanensis suspension cell
biotic stress
chemical constituent
triterpenoid
anti-inflammatory activity
