摘要
目的探讨呼吸道合胞病毒(RSV)抗体检测在早期感染诊断中的应用。方法选择2020年1月—2023年1月平邑县人民医院收治的650例疑似RSV感染者作为研究对象。采用间接荧光免疫法检测RSV,以患者急性期(发热、呼吸困难、发绀,持续数天至数周)与恢复期(症状减轻,炎症消退,持续数周到数月)RSV-免疫球蛋白G(IgG)血清效价升高4倍作为RSV感染的判定标准。结果650例患者中,252例进行了呼吸道合胞病毒抗原(RSV-Ag)检测,检出阳性样本49例,阳性率为19.44%;398例进行了RSV-免疫球蛋白A(IgA)检测,检出阳性样本85例,阳性率为21.36%。以RSV-IgG血清效价升高4倍为阳性判断依据,可见检出RSV-Ag阳性样本41例,符合率为83.67%;检出RSV-IgA阳性样本69例,符合率为84.18%。结论RSV的检出与早期RSV感染有较高的符合率,间接荧光免疫法的检测结果准确可靠,同时检测RSV-Ag与RSV-IgA能够提高RSV感染的早期诊断率。
Objective To analyze the application of respiratory syncytial virus(RSV)antibodies detection for early infection diagnosis.Methods A total of 650 suspected RSV infected individuals from January 2020 to January 2023 admitted in Pingyi County People's Hospital were selected as research objects.RSV was diagnosed by indirect fluorescence immunoassay,and a four-fold increase in serum titer of RSV-immunoglobulin G(IgG)was used to determine RSV infection in patients during the acute phase(fever,dyspnea,cyanosis,lasting several days to several weeks)and the recovery phase(remission of symptoms,resolution of inflammation,lasting several weeks to several months).Results Among the 650 patients,252 cases were tested for RSV-antigen(RSV-Ag),with 49 cases being positive,with a positive rate of 19.44%.The 398 cases were tested for RSV-immunoglobulin A(IgA),with 85 cases being positive,with a positive rate of 21.36%.The results of four-fold increase in serum titer of RSV-IgG in acute stage and convalescent stage were compared,41 cases were RSV-Ag positive,with a coincidence rate of 83.67%.The 69 cases were positive for RSV-IgA,with a coincidence rate of 84.18%.Conclusions The detection of RSV has a high coincidence rate with early RSV infection.The results of indirect fluorescence immunoassay are accurate and reliable,and the simultaneous detection of RSV-Ag and RSV-IgA could improve the early diagnostic rate of RSV infection.
作者
胡少红
Hu Shaohong(Department of Clinical Laboratory,Pingyi County People's Hospital,Linyi 273300,Shandong,China)
出处
《实用检验医师杂志》
2023年第4期397-400,共4页
Chinese Journal of Clinical Pathologist
关键词
呼吸道合胞病毒
感染
阳性率
Respiratory syncytial virus
Infection
Positive rate
