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表面增强拉曼光谱法快速测定凉茶中的甲硝唑 被引量:2

Rapid detection of metronidazole in herbal tea by surface-enhanced Raman spectroscopy
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摘要 目的建立一种表面增强拉曼光谱法(surface-enhanced Raman spectroscopy,SERS)快速检测凉茶中甲硝唑的方法。方法以乙腈为提取剂,以十八烷基键合硅胶固定相和N-丙基乙二胺为净化剂对凉茶样品进行提取净化,获得凉茶样品待测液。利用光还原原理,在太阳光照下通过甲醇还原四氯金酸合成了金纳米颗粒。将合成的金纳米颗粒作为基底材料,对凉茶样品待测液进行表面增强拉曼光谱分析。结果在0.05~3.00g/L范围内,甲硝唑的特征峰强度和浓度具有良好的线性关系,相关系数为0.9991,方法检出限为0.05g/L,方法成功应用于凉茶样品中甲硝唑的检测,回收率为92.1%~102.0%,相对标准偏差为3.8%~8.5%。结论本方法准确度高、操作简单快速,满足凉茶中甲硝唑的快速检测要求,在凉茶中非法添加物现场快速检测方面具有良好的应用潜力。 Objective To establish a method for rapid detection of metronidazole in herbal tea by surface-enhanced Raman spectroscopy(SERS).Methods Using acetonitrile as the extraction agent,octadecyl bonded silica gel fixed phase and primary secondary amines as the purification agents,the herbal tea sample was extracted and purified to obtain the test solution.Additionally,gold nanoparticles were synthesized based on photoreduction synthesis strategy,achieved by reducing tetrachloroauric acid with methanol under sunlight.These synthesized gold nanoparticles served as the base material for SERS analysis in herbal tea samples.Results In the range of 0.05‒3.00 g/L,the characteristic peak intensity and concentration of metronidazole had a good linear relationship,the correlation coefficient was 0.9991,and the limit of detection was 0.05 g/L.The method had been successfully applied to the determination of metronidazole in herbal tea samples,with the recoveries of 92.1%‒102.0%and the relative standard deviations of 3.8%‒8.5%.Conclusion This method has high accuracy,simple and rapid operation,meets the requirements of rapid detection of metronidazole in herbal tea,and has good application potential in the field rapid detection of illegal additives in herbal tea.
作者 林长虹 黄惠 李小苑 彭贵 邓琳娜 申红桃 江宛桐 古丽君 伍聪 江培淳 LIN Chang-Hong;HUANG Hui;LI Xiao-Yuan;PENG Gui;DENG Lin-Na;SHEN Hong-Tao;JIANG Wan-Tong;GU Li-Jun;WU Cong;JIANG Pei-Chun(Shenzhen Academy of Metrology&Quality Inspection,Rapid Food Testing and Examination Division,Shenzhen 518131,China)
出处 《食品安全质量检测学报》 CAS 北大核心 2023年第24期177-183,共7页 Journal of Food Safety and Quality
基金 广东省市场监督管理局科技项目(2022CZ12)。
关键词 表面增强拉曼光谱法 甲硝唑 凉茶 surface-enhanced Raman spectroscopy metronidazole herbal tea
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