摘要
[目的]制备具有良好线性的蔗糖密度梯度溶液。[方法]利用溶液梯度混合仪和蠕动泵组装成新型的蔗糖密度梯度溶液制备器,用该制备器制备50%~0%和50%~10%等不同蔗糖密度梯度溶液,然后利用电脑核酸蛋白检测仪检测所制备的蔗糖密度梯度溶液的光密度,并对蔗糖密度溶液的线性进行评估;比较自制的蔗糖密度梯度溶液制备器与经典的Biocomp全自动密度梯度制备仪制备的蔗糖密度梯度溶液的优劣。[结果]自制的蔗糖密度梯度溶液制备器制备的50%~0%和50%~10%蔗糖密度梯度溶液的光密度与蔗糖密度的决定系数(R^(2))都在0.98以上;Biocomp全自动密度梯度制备仪制备的50%~0%蔗糖密度梯度溶液的光密度与蔗糖密度的决定系数(R^(2))为0.95~0.97。[结论]自制的蔗糖密度梯度溶液制备器制备的蔗糖密度梯度溶液优于Biocomp全自动密度梯度制备仪制备的蔗糖密度梯度溶液。
[Objective]To prepare sucrose density gradient solution with good linearity.[Method]A new sucrose density gradient solution preparator was assembled by utilizing a solution gradient mixer and a Peristaltic pump.This device was utilized to prepare 50%-0%and 50%-10%sucrose density gradient solutions,which were then analyzed by using computer nucleic acid protein detector.Additionally,the linearity of the sucrose density gradient solution prepared by the self-made preparator was compared to that of the classic Biocomp automatic density gradient separation system.[Result]The coefficient of determination(R-squared)of the 50%-0%and 50%-10%sucrose density gradient solutions prepared by the self-made sucrose density gradient solution preparator was found to be above 0.98.In comparison,the coefficient of determination(R-squared)of the 50%-0%sucrose density gradient solution prepared by the Biocomp automatic density gradient separation system was found to be between 0.95-0.97.[Conclusion]The linearity of the sucrose density gradient solution prepared by the self-made sucrose density gradient solution preparation device is superior to that prepared by the Biocomp automatic density gradient separation system.
作者
蒋智文
黄婉玲
林智鑫
杨婉晴
孙雪荣
刘新光
JIANG Zhiwen;HUANG Wanling;LIN Zhixin;YANG Wanqing;SUN Xuerong;LIU Xinguang(Dongguan Key Laboratory for Development and Application of Experimental Animal Resources in Biomedical Industry,Laboratory of Animal Center,Guangdong Medical University,Dongguan 523808,China;Guangdong Provincial Key Laboratory of Medical Molecular Diagnostics,Institute of Aging Research,Guangdong Medical University,Dongguan 523808,China;School of Pharmacy,Guangdong Medical University,Dongguan 523808,China)
出处
《生物技术》
CAS
2023年第6期697-701,共5页
Biotechnology
基金
国家自然科学基金项目(81971329)
广东省基础与应用基础研究基金区域联合基金项目(地区培育项目)(2022A1515140034)
广东省医学科研基金项目(A2021496)
广东医科大学青年培育基金项目(GDMUQ2021002)。
关键词
离心法
蔗糖
密度梯度
全自动密度梯度制备系统
溶液
吸光度
线性拟合
决定系数
centrifuge method
sucrose
density gradient
automatic density gradient separation system
solution
absorbance
lin-ear fitting
coefficient of determination
