摘要
目的分析化学发光免疫法检测糖尿病患者血清胰岛素和C肽水平及胰岛自身抗体的临床价值。方法选取2019年1月至2020年1月辽阳市中心医院收治的85例糖尿病患者纳入研究组,另选取同期80名健康体检者纳入对照组。两组均采用化学发光免疫法检测血清胰岛素和C肽水平,研究组另采用放射免疫法检测血清胰岛素和C肽水平,并采用两种检测方法检测胰岛自身抗体[胰岛细胞抗体(ICA)、糖尿病相关的谷氨酸脱羧酶抗体(GADA)、蛋白质酪氨酸磷酸酶抗体(IA2A)、胰岛素抗体(IAA)、锌抗8抗体(ZnT-8A)]。比较两组血清胰岛素和C肽水平,比较两种检测方法胰岛素、C肽水平及胰岛自身抗体阳性检出率,绘制ROC曲线分析两种检测方法对胰岛自身抗体的诊断价值。结果研究组胰岛素、C肽水平均低于对照组,差异有统计学意义(P<0.05)。化学发光免疫法检测胰岛素、C肽水平均低于放射性免疫法,差异有统计学意义(P<0.05)。化学发光免疫法ICA、GADA、IA2A、IAA、ZnT-8A阳性检出率均高于放射性免疫法,差异有统计学意义(P<0.05)。ROC曲线分析结果显示,化学发光免疫法检测ICA、GADA、IA2A、IAA、ZnT-8A的AUC分别0.872、0.806、0.774、0.640、0.458;放射性免疫法检测ICA、GADA、IA2A、IAA、ZnT-8A的AUC分别为0.567、0.621、0.602、0.527、0.594;化学发光免疫法对5种胰岛自身抗体联合检测的AUC为0.892,大于放射性免疫法的0.635,差异有统计学意义(P<0.05),化学发光免疫法具有一定诊断价值。结论化学发光免疫法可准确检测胰岛素、C肽水平,胰岛自身抗体阳性检出率较高,且对5种胰岛自身抗体联合检测价值较高,对临床辅助诊断和治疗糖尿病具有重要意义。
Objective To analyze the clinical value of chemiluminescence immunoassay in detecting serum insulin and C-peptide levels and islet autoantibodies in patients with diabetes.Methods A total of 85 patients with diabetes admitted to Liaoyang Central Hospital from January 2019 to January 2020 were selected as the study group,and 80 healthy subjects in the same period were selected as the control group.Two groups were tested for serum insulin and C-peptide levels using chemiluminescence immunoassay,study group was tested for serum insulin and C-peptide levels by radioimmunoassay.Two detection methods were used to detect islet autoantibodies(islet cell antibody[ICA],glutamic acid decarboxylase antibody[GADA],protein tyrosine phosphatase antibody[IA2A],insulin antibody[IAA],zinc anti-8 antibody[ZnT-8A]),the levels of serum insulin and Cpeptide were compared between the two groups,the levels of insulin and C-peptide and the positive detection rate of islet autoantibodies were compared between the two detection methods,the ROC curve was drawn to analyze the diagnostic value of the two detection methods for islet autoantibodies.Results The levels of insulin and C-peptide in the study group were significantly lower than those in the control group,the differences were statistically significant(P<0.05).The levels of insulin and C-peptide by chemiluminescence immunoassay were significantly lower than those by radioimmunoassay,and the differences were statistically significant(P<0.05).The positive detection rate of ICA,GADA,IA2A,IAA and ZnT-8A by chemiluminescence immunoassay were higher than those by radioimmunoassay,and the differences were statistically significant(P<0.05).The ROC curve analysis showed that the AUC of ICA,GADA,IA2A,IAA and ZnT-8A detected by chemiluminescence method was 0.872,0.806,0.774,0.640 and 0.458 respectively;the AUC of ICA,GADA,IA2A,IAA and ZnT-8A detected by radioimmunoassay was 0.567,0.621,0.602,0.527 and 0.594 respectively;the AUC of the combined detection of five islet autoantibodies by chemilumin
作者
冯子健
FENG Zijian(Department of Clinical Laboratory,Liaoyang Central Hospital,Shenyang,Liaoning,111000,China)
出处
《当代医学》
2023年第33期84-87,共4页
Contemporary Medicine
