摘要
本研究基于超高效液相色谱-串联质谱(UPLC-MS/MS)结合QuEChERS快速净化方法,旨在建立禽用饲料中苏丹红等6种化工染料的定量分析方法。样品用乙腈提取,C_(18)+PSA净化材料净化,净化液经Hypersil GOLD C_(18)反向色谱柱分离,在电喷雾离子源(ESI)正、负离子双扫描模式下多反应监测(MRM),以标准曲线外标法定量。结果表明:6种化合物线性关系良好;相关系数(R^(2))>0.999,4种苏丹红和酸性橙Ⅱ检出限(LOD)为0.5μg/kg,定量限(LOQ)为1.0μg/kg,罗丹明B LOD为0.05μg/kg,LOQ为0.1μg/kg。添加回收率为90%~110%,相对标准偏差<10%。本研究方法具有快速、灵敏等优势,可实现禽用饲料中6种化工染料的准确定量分析,为禽用饲料的质量安全提供技术参考。
This study was based on the high-performance liquid chromatography-tandem mass spectrometry(UPLC-MS)combined with QuEChERS rapid purification method,a method was developed for the quantitative analysis of sudan,rhodamine B and acid orange Ⅱ in poultry feed.The samples were extracted with acetonitrile and purified with C_(18)+PSA.The purified liquid was separated by Hypersil GOLD C_(18) reversed-phase chromatography column,multi-reaction monitoring(MRM)was performed in an electrospray ionization source(ESI)with positive and negative ion double-scan mode,the quantity was determined by external standard curve method.The results showed that the correlation coefficient(R^(2))>0.999,the detection limits(LOD)of sudan and acid orange Ⅱ were 0.5μg/kg and quantitative limits(LOQ)were 1.0μg/kg,the LOD of rhodamine B were 0.05μg/kg and the LOQ were 0.1μg/kg.The recoveries ranged from 90%~110%,RSD<10%.This study had the advantages of fast and sensitive,it had realized the accurate quantitative analysis of 6 chemical dyes in poultry feed,and provided technical reference for the quality and safety of poultry feed.
作者
李宏
贺习文
晁娟娟
李俊
武治勇
杨森
LI Hong;HE Xiwen;CHAO Juanjuan;LI Jun;WU Zhiyong;YANG Sen(Shaanxi Provincial Animal Husbandry Technology Promotion Station,Xi'an,Shanxi Province 710016,China;Shaanxi Qinyun Agricultural Products Inspection and Testing Co.,Ltd.,Weinan,Shanxi Province 714000,China;Feed Research Institute Chinese Academy of Agricultural Sciences,Beijing 100081,China;Hanzhong Animal Disease Prevention and Control Center,Hanzhong,Shanxi Province 723000,Chinaa)
出处
《中国饲料》
北大核心
2024年第3期118-124,共7页
China Feed
