摘要
为提高水凝胶的负载率和缓释率,以N-羟基丁二酰亚胺和乙基(3-二甲基氨基丙基)碳酰二亚胺盐为催化剂,柠檬酸为交联剂,将丝素蛋白和海藻酸钠交联制备得到丝素蛋白/海藻酸钠水凝胶,测试了水凝胶的结构、形貌、溶失率、吸水率、载药率和释药率。结果显示:丝素蛋白/海藻酸钠水凝胶的最佳制备工艺条件为丝素质量分数2.5%,柠檬酸质量分数2.5%,海藻酸钠质量分数4.5%,催化剂(质量比为1∶1)质量分数为0.5%。红外光谱图显示,在该条件下制备的水凝胶中含有丝素蛋白和海藻酸钠的特征吸收峰,其形貌为多孔结构,24 h后溶失率为11.4%,吸水率约70%,其最大载药率约50%,在pH=7.4的缓冲溶液中缓释8 h后,其释药率接近75%,表现出良好的释药性能。
In order to improve the loading rate and sustained release rate of hydrogels,silk fibroin/sodium alginate hydrogels were prepared by crosslinking reaction between silk fibroin with sodium alginate,using N-hydroxysuccinimide and ethyl(3-dimethylaminopropyl)carbodiimide salt as catalysts and citric acid as crosslinking agent.The structure,morphology,dissolution rate,water absorption,drug loading rate and drug release rate of the hydrogels were tested.The results show that the optimal preparation conditions of silk fibroin/sodium alginate hydrogel are:the mass fraction of silk fibroin 2.5%,the mass fraction of citric acid 2.5%,the mass fraction of sodium alginate 4.5%,and the mass fraction of catalyst(mass ratio 1∶1)0.5%.The infrared spectrum of the hydrogel prepared under this condition contains the characteristic absorption peaks of silk fibroin and sodium alginate.Its morphology is porous.After 24 h,the dissolution rate is 11.4%,the water absorption rate is about 70%,and the maximum drug loading rate is about 50%.In the buffer solution with pH=7.4,the drug release rate is close to 75%after 8 h.It shows good drug release performance.
作者
巢梦颖
凌新龙
刘雨鹭
黄幸菊
林海涛
CHAO Mengying;LING Xinlong;LIU Yulu;HUANG Xingju;LIN Haitao(College of Biological and Chemical Engineering,Guangxi University of Science and Technology,Liuzhou 545006,Guangxi,China;Liuzhou Liujiang District Hospital of Traditional Chinese Medicine,Liuzhou 545199,Guangxi,China)
出处
《纺织高校基础科学学报》
CAS
2023年第6期22-29,共8页
Basic Sciences Journal of Textile Universities
基金
广西科技大学博士基金(17z07)
广西大学生创新创业训练计划资助项目(S202110594013)。
关键词
丝素蛋白
水凝胶
柠檬酸
海藻酸钠
释药性能
silk fibroin
hydrogel
citric acid
sodium alginate
drug release property
