摘要
目的分析血清外泌体环状RNA hsa_circ_0018430在肺鳞状细胞癌(lung squamous cell carcinoma,LSCC)的表达水平与临床意义。方法2021年3月至2022年10月期间收集46例LSCC患者的肿瘤组织与血清样本。提取血清外泌体,通过透射电镜、NTA以及western blot对血清外泌体的形态与粒径分布进行了表征。利用实时荧光定量PCR(quantitative real-time PCR,qPCR)法检测血清外泌体hsa_circ_0018430相对表达水平,采用受试者工作特征(ROC)曲线并结合患者临床特征分析hsa_circ_0018430对LSCC的诊断,采用ELISA检测LSCC患者BICC1蛋白的表达。结果LSCC患者血清外泌体呈具有皮膜结构的球状,粒径分布在30~180 nm之间,血清外泌体hsa_circ_0018430表达水平分布为3.928(2.553~5.303)。ROC分析表明血清外泌体hsa_circ_0018430的AUC值为0.881(95%CI:0.809~0.952,P<0.001),灵敏度86.51%,特异性91.37%。血清外泌体hsa_circ_0018430表达水平与肺鳞癌患者TNM分期、淋巴结转移和血清CEA基线水平有关(P<0.05)。TCGA数据库分析表明LSCC患者组织中BICC1呈低表达(P<0.05),BICC1的表达水平与TNM分期和总生存期无显著相关性。ELISA检测LSCC患者癌组织中BICC1的表达水平相比于癌旁组织显著下调(P<0.05)。结论LSCC患者血清外泌体hsa_circ_0018430表达水平升高,与癌症进展指标相关,有望成为LSCC诊断与治疗评估的新型生物标志物。
Objective To analyze the expression and clinical significance of serum exosomal circular RNA hsa_circ_0018430 in lung squamous cell carcinoma(LSCC).Methods From March 2020 to October 2022,tumor tissues and serum samples were collected from patients with LSCC.Serum exosomes were extracted,and the morphology and particle size distribution of serum exosomes were characterized by transmission electron microscopy,NTA,and western blot.Quantitative real⁃time PCR(qPCR)was used to detect the relative expression of hsa_circ_0018430.The receiver operating characteristic(ROC)curve and clinical significance of hsa_circ_0018430 in serum exosomes were used to analyze the efficacy of hsa_circ_0018430 in diagnosing LSCC.ELISA assays were used to detect the expression of BICC1 in LSCC.Results The relative expression level of serum exosomal hsa_circ_0018430 in LSCC patients was significantly higher than that in healthy controls[3.928(2.553~5.303)vs.1.823(0.798~2.848),P<0.001],sensitivity 86.51%,specificity 91.37%.According to the ROC analysis,the AUC of hsa_circ_0018430 expression for distinguishing LSCC from healthy controls was 0.881(95%CI:0.809~0.952,P<0.001).The level of serum exosomal hsa_circ_0018430 was correlated with TNM stage,lymphatic metastasis and serum CEA level(P<0.05),but not with age,sex,tumor size,and smoking history(P>0.05).The TCGA database analysis showed the significant impaired BICC1 expression in LSCC patients(P<0.05),and there was no significant association between the expression level of BICC1 and TNM stage and overall survival(P>0.05).The expression level of BICC1 in cancer tissues of 46 LSCC patients was significantly down⁃regulated compared with that in adjacent tissues by ELISA assays(P<0.05).Conclusion The serum exosomal hsa_circ_0018430 expression level in LSCC patients is increased,which is expected to be the valuable new biomarkers for the diagnosis and treatment of LSCC.
作者
王志鹏
张倩
黄燕华
孙赟
方晓明
施宇佳
Wang Zhipeng;Zhang Qian;Huang Yanhuan;Sun Yun;Fang Xiaoming;Shi Yujia(Department of Respiratory and Critical Care Medicine,the Affiliated Changzhou NO.2 People′s Hospital of Nanjing Medical University,Changzhou 213003,China)
出处
《中华肺部疾病杂志（电子版）》
2023年第6期774-778,共5页
Chinese Journal of Lung Diseases(Electronic Edition)
基金
常州市卫生健康青苗人才培养工程(CZQM2020077)。
