摘要
枯萎病是危害海岛棉生产的重要因素之一,研究枯萎病抗性分子机制是培育抗病海岛棉品种的分子基础。基于对前期转录组测序数据分析,本研究对参与海岛棉抗枯萎病调控的糖基转移酶基因进行了初步研究,以8个不同抗病性的海岛棉品种为材料,在枯萎病菌处理后,利用实时荧光定量PCR检测9个棉花抗枯萎病相关基因进行表达量分析,结果表明,GB_A03G0575在海岛棉枯萎病侵染过程中随着时间的推移,表达量会先减少再增加,推断其可能在受到病菌胁迫时植物生长会受到影响。GB_A13G1825和GB_A11G1787基因在抗病材料中的表达量会比感病材料的多,最大表达量出现的时间,感、抗材料基本一致,推测这些基因对抗病有一定影响,表明在抗病过程中三个基因可能起着比较重要的作用。本研究结果为海岛棉抗枯萎病育种及抗病机理提供候选基因资源。
Fusarium wilt is one of the important factors endangering the production of island cotton.The study on the molecular mechanism of Fusarium wilt resistance is the molecular basis of breeding island cotton varieties resistant to disease.Based on the early stage of the transcriptome sequencing data analysis,this study involved in the regulation of sea island cotton resistance to blight glycosyl transferase gene is studied,with eight different resistance to sea island cotton varieties as materials,after the Fusarium processing,using real-time fluorescent quantitative PCR detection of nine cotton blight resistance related gene expression quantity analysis,the results showed that the GB_A03G0575 mian flowers wither in the process of the disease infect on the island with the passage of time,the express will reduce first,then increased,inferring the bacteria may be under stress when the plant growth would be affected.GB_A13G1825 and GB_A11G1787 were more expressed in resistant materials than in susceptible materials.The time of the occurrence of the maximum expression was basically the same between susceptible and resistant materials.It was speculated that these genes had a certain influence on disease resistance,indicating that three genes might play a relatively important role in the process of disease resistance.The results of this study provide candidate gene resources for Fusarium wilt resistance breeding and disease resistance mechanism of island cotton.
作者
陆小双
郑凯
龙遗磊
刘鹏飞
韩万里
张梦洁
曲延英
陈全家
邓晓娟
Lu Xiaoshuang;Zheng Kai;Long Yilei;Liu Pengfei;Han Wanli;Zhang Mengjie;Qu Yanying;Chen Quanjia;Deng Xiaojuan(College of Agriculture,Xinjiang Agricultural University,Urumqi,830052)
出处
《分子植物育种》
CAS
北大核心
2024年第2期356-364,共9页
Molecular Plant Breeding
基金
新疆维吾尔自治区青年自然基金项目(2016D01B022)资助。
关键词
海岛棉
枯萎病
糖基转移酶
转录组测序
Island cotton
Fusarium wilt
Glycosyltransferase
Transcriptome sequencing
