摘要
目的:观察电针“足三里”对大肠癌荷瘤小鼠5-氟尿嘧啶(5-FU)化疗后肠黏膜损伤的减轻作用及对结肠组织氧化应激及细胞凋亡的影响,探讨电针缓解化疗后肠黏膜损伤的部分机制。方法:BALB/c小鼠随机分为正常组、CT26组、5-FU组、非穴组和足三里组,每组6只。除正常组外,其余4组采用大肠癌CT26细胞皮下注射种植移植瘤;5-FU组、非穴组和足三里组腹腔注射5-FU(5 mg/mL)溶液,每3 d注射1次,共7次。足三里组和非穴组小鼠在每次给予5-FU腹腔注射后进行电针,足三里组电针双侧“足三里”,非穴组电针双侧非穴,每次5 min,3 d治疗1次,共治疗21 d。治疗结束后评估小鼠腹泻指数,测量小鼠结肠长度;HE染色法观察小鼠结肠黏膜病理形态,测量结肠绒毛长度;生化分析法检测小鼠结肠组织丙二醛(MDA)含量、超氧化物歧化酶(SOD)和谷胱甘肽过氧化物酶(GSH-Px)活性;TUNEL法检测小鼠结肠组织细胞凋亡水平;免疫组织化学法检测小鼠结肠组织B淋巴细胞瘤-2蛋白(Bcl-2)、B淋巴细胞瘤-2基因相关X蛋白(Bax)阳性表达水平。结果:与正常组比较,CT26组小鼠腹泻指数、结肠长度、结肠绒毛长度、MDA含量、SOD活性、GSH-Px活性、细胞凋亡率、Bax、Bcl-2阳性表达率差异均无统计学意义。与CT26组比较,5-FU组小鼠腹泻指数升高(P<0.01),结肠长度及结肠绒毛均缩短(P<0.01),结肠组织MDA含量升高(P<0.05),SOD和GSH-Px活性降低(P<0.01),细胞凋亡率升高(P<0.01),Bax阳性表达升高(P<0.05),Bcl-2阳性表达率降低(P<0.01)。与5-FU组比较,足三里组小鼠腹泻指数降低(P<0.01),结肠长度及结肠绒毛均增长(P<0.01),结肠组织MDA含量降低(P<0.05),SOD和GSH-Px活性升高(P<0.01),细胞凋亡率降低(P<0.01),Bax表达降低(P<0.05),Bcl-2表达升高(P<0.01);非穴组小鼠结肠绒毛长度增长(P<0.01),结肠组织SOD、GSH-Px活性升高(P<0.05,P<0.01),结肠组织细胞凋亡率降低(P<0.01)。结论:电针“足�
ObjectiveTo observe the effect of electroacupuncture(EA)at“Zusanli”(ST36)on intestinal m u⁃cosal dam age,intestinal m ucosal oxidative stress injury and apoptosis induced by 5-fluorouraeil(5-FU)chem otherapy in colorectal cancer-bearing m ice.MethodsThirty m ale BALB/c m ice were random ly divided into norm al control,colorectal cancer(CT26),5-FU,non-acupoint and ST36 groups,with 6 m ice in each group.Except for those of the norm al control group,m ice of the rem aining 4 groups received subcutaneous im plantation of colorectal CT26 cell suspen⁃sion(0.1 m L)in the right arm pit for establishing colorectal cancer m odel.Rats of the 5-FU group,non-acupoint group and ST36 group were given with 5 m g/m L 5-FU solution once every 3 days for a total of 21 days.For m ice of the non-acupoint group and ST36 group,EA(2 Hz,1—2 m A)was applied to bilateral ST36 or non-acupoints(the bilateral sunken spots about 3 m m to the m idpoint between the tail root and the anus)for 5 m in after each intraperitoneal infu⁃sion of 5-FU,once every 3 days,for a total of 21 days.After the intervention,the diarrhea index was assessed.The length of colon(from the endpoint of cecum to the anal orifice)was m easured.Histopathological changes of colonic m u⁃cosa were observed by H.E.staining,and the length of colonic villi was m easured.The content of m alondialdehyde(M DA),and activities of superoxide dism utase(SOD)and glutathione peroxidase(GSH-Px)of colonic tissue were de⁃tected by thibabituric acid,xanthine oxidase and colorim etric m ethod,respectively.The rate of cell apoptosis in the co⁃lonic tissue was m easured by TUNEL assay.The positive expressions of Bax and Bcl-2 in colonic tissue were deter⁃m ined by im m unohistochem istry.ResultsThe CT26 m odel group didn’t show any significant changes in the diarrhea index,colon length,colon villus length,M DA content,SOD and GSH-Px activities,colonic cell apoptosis rate,and Bax and Bcl-2 expression levels when com pared with the norm al group..Com pared with the CT26 group
作者
张学君
林久茂
林晨捷
彭娇
杨晓丹
陈诗兰
赵锦燕
ZHANG Xue-jun;LIN Jiu-mao;LIN Chen-jie;PENG Jiao;YANG Xiao-dan;CHEN Shi-lan;ZHAO Jin-yan(College of Acupuncture and Moxibustion,Fujian University of Traditional Chinese Medicine,Fuzhou 350122,China;College of Integrative Medicine,Fujian University of Traditional Chinese Medicine,Fuzhou 350122;Key Laboratory of Integrative Medicine on Geriatrics of Fujian Province,Fuzhou 350122)
出处
《针刺研究》
CSCD
北大核心
2023年第12期1249-1257,共9页
Acupuncture Research
基金
福建中医药大学针灸学科开放课题(No.XZJ2022001)。
关键词
电针
5-氟尿嘧啶化疗
肠黏膜损伤
足三里
氧化应激
细胞凋亡
Electroacupuncture
5-FU chem otherapy
Dam age of intestinal m ucosa
Zusanli(ST36)
Oxidative stress
Apoptosis
