摘要
目的研究电针足三里对失血性休克(hemorrhagic shock,HS)合并海水浸泡损伤后延迟静脉补液大鼠的平均动脉压(mean arterial pressure,MAP)、心率(heart rate,HR)、乳酸(lactate,LAC)及腹腔脏器血流量的影响。方法选取84只SPF级雄性SD大鼠,随机分为HS+海水浸泡+延迟静脉补液组(对照组)和HS+海水浸泡+电针足三里+延迟静脉补液组(电针组)。大鼠经右侧颈总动脉快速放血,失血量为全身血容量的45%。失血后立即将剑突以下0.5 cm的身体浸泡在(23±1)℃人工海水中30 min。海水浸泡后2 h,回输全部失血及2倍失血量的乳酸林格氏液。电针组于浸泡后0.5 h电针双侧足三里,对照组仅进行固定。监测失血前(基线)、浸泡后即刻、浸泡后2 h、浸泡后5 h和浸泡后24 h的MAP、HR、LAC和腹腔脏器血流量的变化。结果浸泡后即刻,两组大鼠MAP及HR均显著低于失血前(P<0.01);浸泡后2 h,电针组的MAP及HR显著高于对照组(P<0.05)。浸泡后即刻两组大鼠的LAC水平较失血前显著升高(P<0.01);浸泡后5 h,电针组LAC显著低于对照组(P<0.05)。两组大鼠海水浸泡后肾、肝及小肠黏膜血流量比失血前均显著降低(P<0.01)。浸泡后2 h,电针组肾、肝及小肠黏膜血流量较对照组明显改善(P<0.01);浸泡后5 h,电针组肝脏及小肠黏膜血流量仍然显著高于对照组(P<0.05)。结论电针足三里能显著提高失血性休克海水浸泡损伤动物的MAP和HR,降低血LAC水平,改善腹腔脏器血流量,具有一定的抗海战伤休克作用。
Objective To study the effects of electroacupuncture(EA)at Zusanli(ST36)on the mean arterial pressure(MAP),heart rate(HR),lactate(LAC),and blood flow in the abdominal organs of rats with delayed intravenous rehydration after the hemorrhagic shock(HS)and seawater immersion injury.Method Eighty-four SPF-grade male Sprague-Dawley rats were selected and divided into an HS+seawater immersion+delayed intravenous rehydration group(control group)and an HS+seawater immersion+EA at ST36+delayed intravenous rehydration group(EA group).Rapid bloodletting through the right common carotid artery was performed till the blood loss reached 45%of the total blood volume,immediately followed by immersion of the body from 0.5 cm below the xiphoid process in the artificial seawater at(23±1)℃for 30 min.Two hours after seawater immersion,the extracted blood and lactated Ringer’s solution twice of the volume of the blood loss were intravenously infused.The EA group received EA at bilateral Zusanli 0.5 h after seawater immersion,while the control group was only fixed without interventions.Changes in the MAP,HR,LAC and abdominal organ blood flow were monitored before blood loss(the baseline),immediately after immersion,and 2 h,5 h,and 24 h after immersion.Result Immediately after immersion,the MAP and HR were significantly lower in both groups than before blood loss(P<0.01),and the MAP and HR were significantly higher in the EA group than in the control group 2 h after immersion(P<0.05).The LAC level was significantly higher immediately after immersion than before blood loss in both groups(P<0.01),and the LAC level was markedly lower in the EA group than in the control group 5 h after immersion(P<0.05).The blood flow volume in the kidney,liver,and intestinal mucosa decreased significantly in both groups after seawater immersion compared with that before blood loss(P<0.01).The blood flow volume in the kidney,liver,and intestinal mucosa notably improved in the EA group compared with the control group 2 h after immersion(P<0.01),and the
作者
王莉莉
胡森
张海湃
田雨沫
孟祥熙
张慧苹
关玲
WANG Lili;HU Sen;ZHANG Haipai;TIAN Yumo;MENG Xiangxi;ZHANG Huiping;GUAN Ling(Traditional Chinese Medicine Department,The 6th Medical Center of Chinese PLA General Hospital,Beijing 100048,China;Research Center of Trauma Repair and Tissue Regeneration,Medical Innovation Research Division,Chinese PLA General Hospital,Beijing 100037,China;Strategic Support Force Xingcheng Special Duty Sanatorium,Huludao 125105,China;Affiliated Hospital of Chengde medical University,Chengde 067000,China)
出处
《上海针灸杂志》
CSCD
2023年第12期1308-1313,共6页
Shanghai Journal of Acupuncture and Moxibustion
基金
国家自然科学基金项目(81774422)
军队十三五项目(BHJ17J010)。
关键词
电针
穴
足三里
休克
失血性
海水浸泡
大鼠
Electroacupuncture
Point,Zusanli(ST36)
Shock,Hemorrhagic
Seawater immersion
Rats
