摘要
目的基于功能模型对痹祺胶囊进行拆方研究,阐明痹祺胶囊的配伍规律,揭示痹祺胶囊配伍的合理性。方法在各功能实验中,动物分组分别为空白组(抗炎实验和扭体实验除外),模型组(热板实验除外),阳性药组,痹祺胶囊组,益气养血组,活血通络组,抗炎止痛组。益气养血功能实验中,采用环磷酰胺建立大鼠免疫低下模型,测定各给药组对模型大鼠外周血细胞数量、血浆促红细胞生成素(erythropoietin,EPO)含量、脾脏指数及脾脏CD4^(+)/CD8^(+)细胞比例的影响。活血通络功能实验中,采用iv高分子右旋糖苷建立大鼠血循环障碍模型,测定并对比各组肠系膜微循环毛细血管体积流量、全血黏度、凝血酶时间(thrombin time,TT)的影响。在抗炎功能实验中,采用巴豆油建立小鼠耳肿模型,测定各组耳肿胀度及耳肿胀抑制率。在中枢镇痛功能实验中,采用小鼠热板致痛实验法,测定并对比各组末次给药后60、90 min时小鼠舔足反应时间。在外周镇痛功能实验中,采用小鼠醋酸扭体实验法,测定并对比各组发生扭体反应的次数。结果痹祺胶囊与益气养血组可增加气血两虚模型大鼠外周血细胞如白细胞、红细胞及血红蛋白数量(P<0.01),升高血浆EPO含量(P<0.01),降低脾脏指数(P<0.05、0.01),提高脾脏CD4^(+)/CD8^(+)细胞比例(P<0.05、0.01)。痹祺胶囊与活血通络组可升高血循环障碍模型大鼠肠系膜毛细血管体积流量(P<0.01),降低不同切速下全血黏度和红细胞聚集指数(P<0.01),延长血浆TT(P<0.01)。痹祺胶囊与抗炎止痛组可降低耳肿模型小鼠耳肿胀度(P<0.01),可延长正常小鼠给药90 min时热板舔足时间(P<0.01),可抑制醋酸所致小鼠产生扭体反应的次数(P<0.01)。结论益气养血组药党参、茯苓、白术为痹祺胶囊主要发挥益气养血功能的配伍药味;活血通络组药川芎、丹参、三七、地龙、牛膝为痹祺胶囊主要发挥活血通络功�
Objective Based on the function model,Biqi Capsule(痹祺胶囊)was disassembled to study its compatibility law and reveal the rationality of compatibility law of Biqi Capsule.Methods In each functional experiment,the animals used in the study were divided into blank group(except for anti-inflammatory experiment and writhing experiment),model group(except for hot plate experiment),positive drug group,Biqi Capsule group,replenishing qi and nourishing blood group,promoting blood circulation and dredging collaterals group,and anti-inflammatory and analgesia group.In the function experiment of replenishing qi and nourishing blood,the immunosuppressive model of rats was established by intraperitoneal injection of cyclophosphamide,and the effects of each administration group on the number of peripheral blood cells,plasma erythropoietin(EPO)content,spleen index and spleen CD4^(+)/CD8^(+)ratio of the model rats were measured.In the function experiment of promoting blood circulation and dredging collaterals,the rat model of blood circulation disturbance was established by intravenous injection of high molecular dextran,and the effects of mesenteric microcirculation capillary volume flow rate,whole blood viscosity,and thrombin time(TT)were measured and compared in each group.In the anti-inflammatory function experiment,croton oil was used to establish the mouse ear swelling model,and the ear swelling degree and ear swelling inhibition rate of each group were measured.In the central analgesia function experiment,the mouse hot plate pain test method was used to measure and compare the mouse licking reaction time at 60,90 min after the last administration of each group.In the peripheral analgesia function experiment,the times of writhing reaction in each group were measured and compared by acetic acid writhing test method of mice.Results Biqi Capsule group and replenishing qi and nourishing blood group could increase the number of peripheral blood cells such as white blood cell,red blood cell and hemoglobin(P<0.01),increase p
作者
王春芳
李新
张祥麒
姚鹏飞
韩彦琪
王磊
徐旭
许浚
赵专友
张铁军
刘昌孝
WANG Chun-fang;LI Xin;ZHANG Xiang-qi;YAO Peng-fei;HAN Yan-qi;WANG Lei;XU Xu;XU Jun;ZHAO Zhuan-you;ZHANG Tie-jun;LIU Chang-xiao(Tianjin University of Traditional Chinese Medicine,Tianjin 301617,China;Tianjin Key Laboratory of Quality Marker of Traditional Chinese Medicine,Tianjin Institute of Pharmaceutical Research,Tianjin 300462,China;National Key Laboratory of Druggability Evaluation and Systematic Translational Medicine,Tianjin Institute of Pharmaceutical Research,Tianjin 300462,China;National&Local United Engineering Laboratory of Modern Preparation and Quality Control Technology of Traditional Chinese Medicine,Tianjin Institute of Pharmaceutical Research,Tianjin 300462,China;Tianjin Darentang Jingwanhong Pharmaceutical Co.,Ltd.,Tianjin 300112,China;Tianjin Pharmaceutical Da Ren Tang Group Co.,Ltd.,Tianjin 300193,China)
出处
《中草药》
CAS
CSCD
北大核心
2023年第23期7597-7606,共10页
Chinese Traditional and Herbal Drugs
基金
国家自然科学基金重点项目(U21A20406)。
关键词
痹祺胶囊
功能配伍
益气养血
活血通络
抗炎止痛
Biqi Capsule
functional compatibility
replenishing qi and nourishing blood
promoting blood circulation and dredging collaterals
anti-inflammation and analgesia
