摘要
山银花是一种有价值的药用资源,但其油的化学成分和生物活性尚未深入研究。本文通过气相色谱-质谱仪联用法(GC-MS)及超高效液相色谱-高分辨质谱联用(UHPLC-QE-MS)对超临界CO_(2)萃取法得到的山银花油进行系统的化学成分表征,并探讨其体外抗氧化及细胞抗炎能力等生物活性。GC-MS共鉴别出66种化合物,主要为脂肪酸类、甾醇类和烷烃类化合物。UHPLC-QE-MS在正负离子模式下分别鉴别出83和71种化合物,主要为萜类、黄酮类等化合物。抗氧化结果表明山银花油清除ABTS自由基能力呈良好的剂量依赖性,DPPH自由基的IC_(50)值为3.49 mg/mL。同时抗炎实验表明山银花油可显著抑制脂多糖(LPS)诱导小鼠巨噬细胞(RAW 264.7)炎性因子IL-6(P<0.05)和NO(P<0.05)的释放,发挥抗炎作用。以上结果表明,山银花油化学成分复杂多样,是一种具有较优抗炎作用的天然活性来源,且在食品医药中有较好的开发和应用价值。
Lonicerae Flos is a valuable medicinal resource,but the chemical composition and biological activity of its oil have not been studied in depth.In this study,the Lonicerae Flos oil was prepared by supercritical CO_(2) extraction method.A systematic characterization of chemical components was performed based on gas chromatography-mass spectrometry(GC-MS)and ultra-high performance liquid chromatography-high resolution mass spectrometry(UHPLC-QE-MS).Its biological activities such as anti-inflammatory and antioxidant activities in vitro were also studied.The GC-MS results based on non-methylation and methyl esterification methods indicated a total of 66 chemicals and the main compounds were fatty acids,sterols,and alkanes.UHPLC-QE-MS identified 83 and 71 compounds in positive and negative ion modes,respectively,mainly including terpenoids and flavonoids.Moreover,antioxidant activity analysis indicated that the ability of Lonicerae Flos oil to scavenge ABTS free radical has a good dose-dependent,and the IC_(50) of Lonicerae Flos oil involved in the DPPH free radical scavenging assay was 3.49 mg/mL.Meanwhile,The anti-inflammatory test showed that oil can significantly inhibit the expression of IL-6(P<0.05)and NO(P<0.05)levels in RAW 264.7 cells induced by LPS.These results show that the Lonicerae Flos oil is a natural active source with good anti-inflammatory and antioxidant activities,and it has good development and application value in Food and medicine.
作者
刘恃君
陆玉爱
李利
汤杨黔南
林丽美
谢明霞
夏伯候
LIU Shi-Jun;LU Yu-ai;LI Li;TANG Yang-qian-nan;LIN Li-mei;XIE Ming-xia;XIA Bo-hou(Key Laboratory for Quality Evaluation of Bulk Herbs of Hunan Province,College of Pharmacy,Hunan Universityof Chinese Medicine,Changsha 410208,China)
出处
《天然产物研究与开发》
CAS
CSCD
2023年第11期1842-1856,共15页
Natural Product Research and Development
基金
国家重点研发计划中医药现代化研究重点专项(2017YFC1701900)
湖南省自然科学基金(2022JJ50008)
长沙市科技计划(kq2004054)
湖南中医药大学中药学一流学科(2018-3)。
