摘要
目的观察氧化石墨烯对脂肪间充质干细胞增殖和迁移能力的影响及二者联合应用于糖尿病创面的愈合效果。方法采用噻唑蓝(MTT)法验证氧化石墨烯材料的细胞毒性。采用5-乙炔基-2′脱氧尿嘧啶核苷(EdU)实验、细胞划痕实验评估氧化石墨烯对脂肪间充质干细胞增殖、迁移的影响。建立糖尿病创面动物模型,将氧化石墨烯共培养的脂肪间充质干细胞注射到糖尿病裸鼠的创面皮肤边缘,通过LB983活体成像系统观察氧化石墨烯对脂肪间充质干细胞在糖尿病创面上的存活能力的影响。在注射后的第1、7和14天观察创面的愈合情况。通过H-E染色验证氧化石墨烯共培养后的脂肪间充质干细胞对裸鼠糖尿病创面愈合效果的影响。结果细胞实验表明高糖环境中培养的脂肪间充质干细胞在加入氧化石墨烯后表现出更强的增殖和迁移能力。在动物实验中,氧化石墨烯和脂肪间充质干细胞共培养可以提高脂肪间充质干细胞糖尿病创面的存活能力并显著提升创面愈合率。结论氧化石墨烯可以促进糖尿病创面上脂肪间充质干细胞的增殖和迁移,是治疗糖尿病创面的良好辅助材料。
Objective To observe the effect of graphene oxide on the proliferation and migration of adipose-derived mesenchymal stem cells,and the effect of the combined use of graphene oxide and adipose-derived mesenchymal stem cells on diabetic wounds.Methods The cytotoxicity of graphene oxide was examined by MMT assay.The effect of gra-phene oxide on the proliferation and migration of adipose-derived mesenchymal stem cells was evaluated by EdU assay and would healing assay.Then,a diabetic wound model of nude mice was established,and the successfully modeled mice were injected with adipose-derived mesenchymal stem cells at the edge of the wounds.The effect of graphene oxide on the survival of adipose-mesenchymal stem cells was detected by the LB983 in vivo imaging system.The healing of the wounds was observed on days 1,7 and 14 after injection.The healing effect of adipose-mesenchymal stem cells co-cul-tured with graphene oxide on the wounds of diabetic nude mice was verified by hematoxylin and eosin(H-E)staining.Results According to cell experiments,adipose-derived mesenchymal stem cells cultured in a high-glucose environ-ment exhibited stronger proliferation and migration abilities after exposure to graphene oxide.In animal experiments,gra-phene oxide co-cultured with adipose-derived mesenchymal stem cells improved the survival rate of adipose-derived mesenchymal stem cells in the diabetic wounds of nude mice.Conclusions Graphene oxide can promote the proliferation and migration of adipose mesenchymal stem cells on diabetic wounds,which is a good auxiliary material for treating dia-betic wounds.
作者
袁佳琪
纪哲
李强
龙薪竹
金培生
YUAN Jiaqi;JI Zhe;LI Qiang;LONG Xinzhu;JIN Peisheng(Department of Plastic Surgery,the Affiliated Hospital of Xuzhou Medical University,Xuzhou,Jiangsu 221002,China)
出处
《徐州医科大学学报》
CAS
2023年第12期859-868,共10页
Journal of Xuzhou Medical University
基金
国家自然科学基金(82172224)
徐州市卫生健康委青年项目(XWKYHT20220145)。
关键词
氧化石墨烯
脂肪间充质干细胞
干细胞疗法
糖尿病创面
愈合
graphene oxide
adipose-derived mesenchymal stem cells
stem cell therapy
diabetic wounds
healing
