摘要
目的分析高危产妇剖宫产后切口感染外周血分化抗原36(CD36)/哺乳动物雷帕霉素靶蛋白复合物1(mTORC1)通路表达及细胞免疫炎症因子水平。方法选取2018年6月-2022年6月在本院行剖宫产的107例高危产妇,根据产后是否发生切口感染分为感染组21例和未感染组86例。分析感染组切口分泌物培养菌种分布特征,logistic回归分析影响剖宫产后切口感染的危险因素;采用蛋白免疫印迹法(WB)检测外周血中CD36/mTORC1通路相关蛋白[CD36、Toll样受体4(TLR4)、黏着斑激酶(FAK)、mTORC1]表达水平,采用酶联免疫吸附反应(ELISA)检测外周血中细胞免疫炎症因子[白介素-1(IL-1)、白介素-6(IL-6)、白介素-8(IL-8)、肿瘤坏死因子-α(TNF-α)]水平。采用Spearman相关性分析外周血中CD36、mTORC1蛋白表达与细胞免疫炎症因子的相关性。结果切口感染分离菌株以革兰阴性菌(67.44%)为主;logistic回归分析,手术时间≥60min、抗生素使用种类≥3种、侵入性操作≥3次均为影响剖宫产后切口感染的独立危险因素(P<0.01);感染组外周血CD36/mTORC1通路相关蛋白以及免疫炎症因子水平高于未感染组(P<0.05),且外周血IL-1、IL-6、IL-8、TNF-α水平与CD36、mTORC1蛋白表达水平均成正相关关系(P<0.001)。结论高高危产妇剖宫产后切口感染外周血CD36/mTORC1通路表达明显增加,且与细胞免疫炎症因子水平成正相关关系。
Objective This paper aims to analyze the pathway expression of peripheral blood cluster of differentiation 36(CD36)/mammalian target of rapamycin complex 1(mTORC1)and cellular immune inflammatory factors in high-risk puerpe-rae with incision infection after cesarean section.Methods A total of 107 high-risk puerperae undergoing cesarean section in the hospital were enrolled between June 2018 and June 2022.According to presence or absence of incision infection,they were divided into infection group(21 cases)and non-infection group(86 cases).The distributions of cultured bacteria from inci-sion secretions in infection group were analyzed.The risk factors of incision infection after cesarean section were analyzed by lo-gistic regression analysis.The levels of peripheral blood CD36/mTORCI pathways related proteins[CD36,Tll-like receptor 4(TLR4),focal adhesion kinase(FAK),mTORC1] were detected by Western blot(WB).The levels of peripheral blood cellular immune inflammatory factors[interleukin-1(IL-1),interleukin-6(IL-6),interleukin-8(IL-8),tumor nec-rosis factor-α(TNF-α)] were detected by enzyme-linked immunosorbent assay(ELISA).The correlation between periph-eral blood CD36,mTORC1 and cellular immune inflammatory factors was analyzed by Spearman correlation analysis.Results The main pathogen of incision infection was Gram-negative bacteria(67.44%).Logistic regression analysis showed that operation time≥60 min,usage types of antibiotics≥3 and invasive operation≥3 times were independent risk factors for incision infection after cesarean section(P<0.01).The levels of peripheral blood CD36/mTORC1 pathways related proteins and immune inflammatory factors in infection group were higher than those in non-infection group(P<0.05).The levels of peripheral blood IL-1,IL-6,IL-8 and TNF-αwere positively correlated with the levels of CD36 and mTORCI proteins(P<0.001).Conclusion The expression of peripheral blood CD36/mTORCl pathways increase in high-risk puerperae with incision infection after cesarean section,which are pos
作者
莫匡颖
姚丽娜
顾柳芬
MO Kuang-ying;YAO Li-na;GU Liu-fen(Xiaoshan Hospital Afiliated to Hangzhou Normal University,Zhejiang 311202,China)
出处
《中国卫生检验杂志》
CAS
2023年第23期2881-2885,共5页
Chinese Journal of Health Laboratory Technology
关键词
高危产妇
剖宫产
切口感染
分化抗原36
哺乳动物雷帕霉素靶蛋白复合物1
细胞免疫炎症因子
High-risk puerpera
Cesarean section
Incision infection
Cluster of differentiation 36
Mammalian target of rapa-mycin complex 1
Cellular immune inflammatory factor
