摘要
目的研究黄连素是否通过Toll样受体的炎症信号通路改善血糖波动诱导的内皮损伤。方法2022年7月—2023年1月在辽宁沈阳选取雄性Wistar大鼠20只,采用随机数表法分为对照组、波动血糖组、黄连素高剂量组[100 mg/(kg·d)]、黄连素低剂量组[25 mg/(kg·d)],每组5只。应用Ellisa法检测肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)、Toll样受体4(toll-like receptor 4,TLR-4)和天冬氨酸特异性半胱氨酸蛋白酶-3(cysteinyl aspartate-specific proteinase-3,Caspase-3)。结果波动血糖组的TNF-α(30.05±10.65)pg/mL、TLR-4(123.88±19.72)ng/mL、Caspase-3(4.5±0.99)pmol/L表达高于对照组的TNF-α(18.06±4.86)pg/mL、TLR-4(86.28±19.85)ng/mL、Caspase-3(3.36±0.63)pmol/L,差异有统计学意义(P<0.05)。应用高剂量黄连素后的TNF-α(19.28±5.75)pg/mL、TLR-4(89.19±20.02)ng/mL、Caspase-3(3.62±0.51)pmol/L和应用低剂量黄连素后的TNF-α(20.22±6.22)pg/mL、TLR-4(91.41±29.50)ng/mL、Caspase-3(3.63±0.15)pmol/L和波动血糖组比较,差异有统计学意义(P<0.05)。结论血糖波动可增强主动脉内皮细胞中的炎症反应,启动凋亡,应用黄连素后可改善。
Objective To investigate whether berberine can ameliorate blood glucose fluctuation-induced endothelial injury through the inflammatory signaling pathway of Toll-like receptors.Methods 20 male Wistar rats were selected from July 2022 to January 2023 in Shenyang,Liaoning province,and divided into control group,fluctuating blood glucose group,berberine high-dose group[100 mg/(kg·d)]and berberine low-dose group[25 mg/(kg·d)]by random number table method,with 5 rats in each group.Application of Ellisa method for detecting tumor necrosis factor-α(tuber crossing factor-α,TNF-α)、toll like receptor 4(TLR-4)and aspartate specific protease-3(Caspase-3).Results The expressions of TNF-α(30.05±10.65)pg/mL,TLR-4(123.88±19.72)ng/mL and Caspase-3(4.5±0.99)pmol/L in the fluctuated blood glucose group were higher than those in the control group(18.06±4.86)pg/mL and TLR-4(86.28±19.85)ng/mL,Caspase-3(3.36±0.63)pmol/L,and the differences were statistically significant(P<0.05).TNF-α(19.28±5.75)pg/mL,TLR-4(89.19±20.02)ng/mL,and Caspase-3(3.62±0.51)pmol/L after the application of berberine high-dose group,and TNF-α(20.22±6.22)pg/mL,TLR-4(91.41±29.50)ng/mL,and Caspase-3(3.63±0.15)pmol/L after the application of berberine low-dose group,were compared with those in fluctuating blood glucose group,and the differences were statistically significant(P<0.05).Conclusion Blood glucose fluctuation can enhance the inflammatory response and initiate apoptosis in aortic endothelial cells,which can be improved by the application of berberine.
作者
张微
彭观景
黄春
李青
黄云飞
李洁
周波
刘雅雯
李艳
ZHANG Wei;PENG Guanjing;HUANG Chun;LI Qing;HUANG Yunfei;LI Jie;ZHOU Bo;LIU Yawen;LI Yan(Department of Endocrinology,Zhanjiang Central People's Hospital,Zhanjiang,Guangdong Province,524000 China;Department of Liver Surgery,Zhanjiang Central People's Hospital,Zhanjiang,Guangdong Province,524000 China;Department of Emergency Medicine,Zhanjiang Central People's Hospital,Zhanjiang,Guangdong Province,524000 China;Department of Dry Diagnosis,the First Affiliated Hospital of China Medical University,Shenyang,Liaoning Province,110000 China)
出处
《系统医学》
2023年第18期37-40,共4页
Systems Medicine
基金
广东省中医药局科研项目(20211443)
湛江市非资助科技攻关计划项目(2020B01345)
湛江中心人民医院院级科研项目(2020A13)。
关键词
血糖波动
内皮细胞
炎症
黄连素
Blood glucose fluctuation
Endothelial cells
Inflammation
Berberine
