摘要
以广佛手为原料,探究热水提取法(H)、高温蒸煮辅助热水浸提法(HTH)、超微粉碎辅助热水浸提法(UMH)、复合酶解法(E)、高温蒸煮辅助复合酶解法(HTE)和超微粉碎辅助复合酶解法(UME)六种方法对其膳食纤维性质的影响。结果表明:H-TDF的纯度(82.49 g/100 g)最高;E-TDF(59.15%)、UMH-IDF(48.45%)和HTE-SDF(23.68%)得率最高;结构方面,六种IDF和SDF均具有多糖特征结构,均为典型纤维素I型结构,均具有相似的表面结构;除H-SDF外,其余SDF的大分子量组分降解。理化性质方面,HTE-IDF的持水力(8.37 g/g)、持油力(2.11 g/g)、阳离子交换能力(0.24 mol/g)最高,而E-IDF的膨胀力(9.89 mL/g)最高;E-SDF的持水力(9.69 g/g)、膨胀力(7.42 mL/g)最高,UME-SDF的持油力(13.76 g/g)最高,E-SDF的阳离子交换能力(0.31 mol/g)最高。对于亚硝酸盐吸附能力,pH值2时UMH-IDF(7.28 mg/g)和H-SDF(3.80 mg/g)最高,pH值7时HTH-IDF(12.87 mg/g)和UME-SDF(1.55 mg/g)最高。综合分析,高温蒸煮辅助复合酶解法总体优于其他方法,且SDF得率最高,可推广应用。
The effects of six extraction methods on the properties of dietary fiber from bergamot were investigated,including hot water extraction(H),high temperature cooking-assisted hot water extraction(HTH),ultra-fine grinding-assisted hot water extraction(UMH),complex enzymolysis with cellulase and xylanase(E),high temperature cooking-assisted complex enzymolysis(HTE),and ultra-fine comminution-assisted complex enzymolysis(UME).The purity of H-TDF(82.49 g/100 g)was the highest.The highest yields were from E-TDF(59.15%),UMH-IDF(48.45%)and HTE-SDF(23.68%)respectively.In terms of structural properties,all IDF and SDF had characteristic polysaccharide structures,typical cellulose I-type structures,and similar surface structures.Except for H-SDF,the macromolecular components of other SDFs were degraded.In terms of physical and chemical properties,HTE-IDF had the highest water holding(8.37 g/g),oil holding(2.11 g/g),and cation exchange capacity(0.24 mol/g),whereas E-IDF had the highest expansion capacity(9.89 mL/g).The water holding(9.69 g/g)and expansion capacity(7.42 mL/g)of E-SDF were the highest,the oil holding capacity(13.76 g/g)of UME-SDF was the highest,and the cation exchange capacity(0.31 mol/g)of E-SDF was the highest.For nitrite adsorption capacity,UMH-IDF(7.28 mg/g)and H-SDF(3.80 mg/g)were the highest at pH value 2,and HTH-IDF(12.87 mg/g)and UME-SDF(1.55 mg/g)were the highest at pH value 7.High temperature cooking-assisted complex enzymolysis was generally superior to other methods,and SDF yield was the highest.This method could be popularized.
作者
华建新
卓思雨
田嘉瑜
郭彦希
周爱梅
HUA Jianxin;ZHUO Siyu;TIAN Jiayu;GUO Yanxi;ZHOU Aimei(Guangdong Provincial Key Laboratory of Nutraceuticals and Functional Foods,College of Food Science,South China Agricultural University,Guangzhou 510642,China)
出处
《现代食品科技》
CAS
北大核心
2023年第12期234-245,共12页
Modern Food Science and Technology
基金
“广东省“扬帆计划”引进创新创业团队项目(2016YT03S056)。
关键词
广佛手
膳食纤维
提取方法
bergamot
dietary fiber
extraction method
