摘要
【目的】克隆了卷丹百合Lilium lancifolium珠芽中LlARR1并对其进行基因表达分析,为分析LlARR1基因在卷丹珠芽形成过程中的作用机制奠定基础。【方法】通过分析珠芽形成过程的转录组数据,筛选到一个注释为ARR1的差异表达的转录本。通过PCR技术,以卷丹混合叶腋cDNA为模板克隆了ARR1的同源基因,并利用相关生物信息学软件对该基因编码的蛋白质结构进行预测;通过荧光定量PCR(qRT-PCR)技术分析了LlARR1在珠芽发生发育过程中的表达模式,并探究了其对不同激素处理的响应。【结果】LlARR1的CDS全长为2148 bp,编码715个氨基酸,不存在跨膜运输结构,属于B型ARR蛋白家族。qRT-PCR结果显示,LlARR1在S0、S1时期表达趋势与转录组数据相一致。LlARR1在卷丹百合叶腋处表达量最高,且在S0时期植株茎秆上部表达量显著高于茎秆下部。同时,其在自然发生珠芽的百合品种中叶腋处的表达量显著高于自然条件下不可发生珠芽的百合品种。说明LlARR1主要在叶腋处发挥功能,并可能在卷丹珠芽发生过程中具有正调控作用。LlARR1的表达在S2(珠芽绿球期)有一个小峰值,推测其在珠芽发育过程中也发挥作用。与对照植株相比,IAA、GA3处理均显著抑制了LlARR1在S0时期的表达,但NPA处理缓解了IAA对LlARR1的抑制;GA3处理在后期的发育过程中促进其表达。推测LlARR1可能参与了生长素和赤霉素调控的珠芽发生发育途径。【结论】LlARR1表达模式分析发现,LlARR1在卷丹珠芽准备发生时期表达水平较高,且可以在叶腋处大量表达,表明LlARR1可能参与了卷丹珠芽的发生和发育,并在该过程中发挥作用。
【Objective】In this study,we cloned LlARR1 from Lilium lancifolium and analyzed its gene expression,which laid a foundation for analyzing the mechanism of LlARR1 gene in the formation of Lilium lancifolium.【Method】A differentially expressed transcript annotated as ARR1 was selected by analyzing the transcriptome data of the process of bead formation.The homologous gene of ARR1 was cloned by PCR using the hybrid axillary cDNA as template,and the structure of the protein encoded by the gene was predicted by bioinformatics software.Quantitative fluorescence PCR(qRT-PCR)was used to analyze the expression pattern of LlARR1 during the development of granulation and its response to different hormone treatments.【Result】The total length of CDS of LlARR1 is 2148 bp,encoding 715 amino acids,and there is no transmembrane transport structure,which belongs to the B-type ARR protein family.The results of qRT-PCR showed that the expression trend of LlARR1 in S0 and S1 peri-ods was consistent with the transcriptome data.The expression level of LlARR1 was highest in the axil-lary area of the Lilium lancifolium,and the expression level in the upper part of the stem was signifi-cantly higher than that in the lower part of the stem during S0 period.Meanwhile,the expression level in the axillary area of the lily varieties with naturally occurring bead buds was significantly higher than that of the lily varieties without bead buds under natural conditions,indicating that LlARR1 mainly ex-erts its function in the axillary area.It may play a positive regulatory role in the process of sprouting.The expression of LlARR1 has a small peak in S2(bud green bulb stage),suggesting that LlARR1 may also play a role in bud development.Compared with control plants,IAA and GA3 treatments signifi-cantly inhibited the expression of LlARR1 at S0 stage,but NPA treatment alleviated the inhibition of IAA on LlARR1.GA3 treatment promoted its expression during later development,suggesting that LlARR1 may be involved in the growth hormone and gibbe
作者
陈妍竹
张雪敏
俞诗音
王梦迪
杜运鹏
杨凤萍
张秀海
李玉舒
曹丽
CHEN Yanzhu;ZHANG Xuemin;YU Shiyin;WANG Mengdi;DU Yunpeng;YANG Fengping;ZHANG Xiuhai;LI Yushu;CAO Li(Agriculture College of Yanbian University,Yanji 133002,China;Institute of Grassland,Flowers and Ecology,Beijing Academy of Agriculture and Forestry Sciences,Beijing 100097,China;School of Landscape Architecture,Beijing Forestry University,Beijing 100083,China;Beijing Vocational College of Agriculture,Beijing 102442,China)
出处
《河南农业大学学报》
CAS
CSCD
2023年第6期984-995,共12页
Journal of Henan Agricultural University
基金
北京市农林科学院科技创新能力建设专项项目(KJCX20230801)
北京市教育委员会科技计划一般项目(KM202112448004)
北京市农林科学院优秀青年科学基金课题项目(YXQN202303)。
关键词
卷丹
珠芽形成
生物信息学分析
表达分析
LlARR1
Lilium lancifolium
bud formation
bioinformatics analysis
expression analysis
LlARR1
