摘要
目的:从DJ-1蛋白调节线粒体复合体I活性的角度,探讨DJ-1介导白藜芦醇(RES)预处理对抗大鼠心肌缺血再灌注(I/R)所诱发的氧化应激损伤的分子机制。方法:心肌内注射DJ-1敲减(sh-DJ-1)或阴性对照(NC)慢病毒后,通过结扎大鼠冠状动脉左前降支法构建心肌I/R模型。将SD大鼠随机分为6组:假手术(sham)组、I/R组、RES+I/R组、NC+RES+I/R组、sh-DJ-1+RES+I/R组和I_(A)CS-010759(线粒体复合体I抑制剂)+RES+I/R组,每组10只。在心肌I/R模型前7 d通过灌胃给予RES(20 mg/kg),每日一次。此外,sham和I/R组大鼠均通过灌胃接受等体积的生理盐水。TTC染色和超声心动图分别观察心肌梗死面积和心功能变化情况;MitoSOX荧光探针检测心肌中线粒体活性氧(ROS)水平;试剂盒检测血清中丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性和乳酸脱氢酶(LDH)活性;结合Western blot和免疫共沉淀实验观察DJ-1与线粒体复合体I的两个亚基ND-1和NDUFA4的相互作用。结果:与I/R组相比,RES预处理可显著降低心肌梗死面积、心脏组织中线粒体ROS水平,以及血清中LDH活性和MDA含量(P<0.01),提高左室射血分数、左室短轴缩短率和血清中SOD活性(P<0.01),增加DJ-1的表达及DJ-1的线粒体转位(P<0.01),促进DJ-1与ND-1/NDUFA4的相互作用进而保护线粒体复合体I的活性(P<0.01)。然而,与RES+I/R组相比,敲减DJ-1的表达后,RES减轻心肌I/R损伤的作用被显著抑制(P<0.05或P<0.01)。结论:RES预处理可增加DJ-1的表达和线粒体易位,促进DJ-1与线粒体复合体I的ND1和NDUFA4亚基的相互作用,从而保护线粒体复合体I的活性并减轻心肌I/R所诱发的氧化应激损伤。
AIM:From the perspective of regulating mitochondrial complex I activity by DJ-1 protein,this study aims to explore the mechanism of DJ-1-mediated resveratrol(RES)preconditioning in protecting against oxidative stress injury induced by myocardial ischemia-reperfusion(I/R)in rats.METHODS:After intramyocardial injection of lentivirus carrying DJ-1 shRNA(sh-DJ-1)or negative control(NC)shRNA,the myocardial I/R model was constructed by ligating the left anterior descending branch of the rat coronary artery.Sprague-Dawley(SD)rats were randomly divided in-to 6 groups:sham group,I/R group,RES+I/R group,NC+RES+I/R group,sh-DJ-1+RES+I/R group,and I_(A)CS-010759(mitochondrial complex I inhibitor)+RES+I/R group,with 10 rats in each group.The rats in RES treatment groups were given RES(20 mg/kg)via gavage for 7 d prior to the myocardial I/R modeling,once daily.Moreover,the rats in sham and I/R groups received an equivalent volume of normal saline via gavage.Myocardial infarction area and cardiac function were assessed by TTC staining and echocardiography,respectively.The MitoSOX fluorescent probe was used to detect levels of mitochondrial reactive oxygen species(ROS)in the myocardium.The levels of malondialdehyde(MDA),superoxide dis-mutase(SOD)and lactate dehydrogenase(LDH)in the serum were detected using kits.Western blot and co-immunopre-cipitation assays were used to observe the interaction between DJ-1 and the two subunits,ND-1 and NDUFA4,of the mito-chondrial complex I.RESULTS:Compared with I/R group,RES pretreatment significantly reduced the myocardial in-farction area,mitochondrial ROS levels,serum LDH activity,and serum MDA content(P<0.01).It also elevated left ventricular ejection fraction,left ventricular fractional shortening and serum SOD activity(P<0.01).Pretreatment with RES increased the expression and mitochondrial translocation of DJ-1(P<0.01),promoted the interaction between DJ-1 and ND-1/NDUFA4,which in turn protected the activity of mitochondrial complex I(P<0.01).However,when the ex-pression of DJ-1 was su
作者
任建民
刘慧茹
刘松
李晓琪
何康
汤蕾
陈和平
REN Jianmin;LIU Huiru;LIU Song;LI Xiaoqi;HE Kang;TANG Lei;CHEN Heping(Jiangxi Provicial Key Laboratory of Basic Pharmacology,School of Pharmaceutical Science,Nanchang University,Nan-chang 330006,China)
出处
《中国病理生理杂志》
CAS
CSCD
北大核心
2023年第12期2185-2192,共8页
Chinese Journal of Pathophysiology
基金
国家自然科学基金资助项目(No.81960586)。
