摘要
【目的】果实颜色是影响茄子商品价值的重要性状。通过解析两白果亲本杂交构建的F2代群体紫红果单株和白果单株特殊分离比产生的原因,为阐明茄子果实颜色形成的调控机制奠定基础。【方法】以花青素合成结构基因ANS(P)突变的白花白果母本19141、未知突变基因位点的白花白果父本19142及其紫红果F1、果色分离的F2群体为试验材料,探讨上位基因控制的茄子果色遗传规律;克隆已知的茄子果色相关D(SmMYB1)和Y(SmDFR)基因,探明父本19142突变的果色基因和方式;开发基因内分子标记,对F2进行基因分型以及与其他果皮无花青素沉着茄子亲本杂交验证,解析上位基因调控茄子果色遗传的分子基础。【结果】E4450 F2紫红果和白果单株分离比符合3对上位基因控制的27﹕37的分离比率,19141的P基因位点发生突变,基因型为DDppYY,19142的D和Y基因位点同时发生了突变,基因型为ddPPyy。克隆测序发现,19142的SmMYB1发生可变剪接,导致第2外显子跳跃。19142的SmDFR起始密码子上游-326 bp的SNP(C→G),导致启动子缺失了1个CAAT-box顺式作用元件;在第2外显子最后一个碱基G变C,发生剪切位点突变。基于SmMYB1、SmANS和SmDFR遗传变异,开发了基因内分子标记对E4450 F2单株进行基因分型,结果发现基因型与表型完全吻合;D_P_Y_对应紫花紫红果表型,ddP_Y_基因型对应紫花白果表型,D_ppY_、D_P_yy、D_ppyy、ddppY_、ddP_yy和ddppyy基因型对应白花白果表型。19142与白果自交系19147(dtdtPPYY)和绿果自交系19144(DDPPyy)分别杂交,结果发现F1果色分别为白色和绿色,果皮无花青素沉着,这进一步证明了19142是SmMYB1和SmDFR两个基因同时发生突变。【结论】2个果皮无花青素沉着的茄子亲本杂交,如果F1代果皮有花青素沉着,且F2代有花青素沉着单株和无花青素沉着果色单株分离比符合27﹕37,则是由于其中的一个亲本在花青素合成通路的1个�
【Objective】Fruit color is an important trait that affects the commercial value of eggplant fruit.By analyzing the causes for the special segregation ratio of individual plants with purple red peel and with white peel in the F2 population constructed by crossing between two white-fruit parents,this paper could lay the foundation for elucidating the mechanism of epistatic gene interaction on regulating eggplant fruit coloration.【Method】The white-flower and white-peel female parent 19141 with mutation at the structural gene ANS involving in the anthocyanin biosynthesis pathway,white-flower and white-peel male parent 19142 with unknown mutation genes involving in the anthocyanin biosynthesis pathway,and their F1 population with purple red peel and F2 population with separate peel colors were used to explore the epistatic inheritance of eggplant fruit coloration.Genes and their mutation patterns were studied by cloning the known genes,D(SmMYB1)and Y(SmDFR)related to peel color in male parent 19142.Molecular basis of peel color-controlling epigenetic genes was analyzed by developing molecular markers based on genetic variations of peel color genes,analyzing the relationship between genotype and phenotype in E4450F2 population,and crossing with other eggplant parents without anthocyanin pigmentation in peel.【Result】The segregation ratio of plants with purple red fruit and white fruit in E4450 F2 progeny was consistent with the segregation ratio of 27:37 controlled by three pairs of epistatic genes,that is,mutations occurred at the P gene locus in 19141,with genotype DDppYY,and mutations occurred at both D and Y gene loci in 19142,with genotype ddPPyy.The results of cloning and sequencing showed that alternative splicing occurred in SmMYB1 of 19142,which led to the second exon skipping.In 19142,SNP(C→G)in the promoter region’s-326 bp upstream of the start codon resulted in the absence of a CAAT-box cis-acting element in SmDFR gene.An SNP,G to C,at the last base of the second exon,was annotated as splicing
作者
陈锦溶
吕紫见
范丽莎
游倩
李涛
宫超
孙光闻
李植良
孙保娟
CHEN JinRong;LÜZiJian;FAN LiSha;YOU Qian;LI Tao;GONG Chao;SUN GuangWen;LI ZhiLiang;SUN BaoJuan(Vegetable Research Institute,Guangdong Academy of Agricultural Sciences/Guangdong Key Laboratory for New Technology Research of Vegetables,Guangzhou 510640;College of Horticulture,South China Agricultural University,Guangzhou 510642;Key Laboratory of Horticultural Plant Biology,Ministry of Education,Huazhong Agricultural University,Wuhan 430070)
出处
《中国农业科学》
CAS
CSCD
北大核心
2023年第23期4729-4741,共13页
Scientia Agricultura Sinica
基金
广西科技重大专项(桂科AA22068088)
广东省现代农业产业技术体系(2023KJ110,2023KJ106)
广东省级乡村振兴战略专项资金种业振兴项目(2022-NPY-00-026)。
关键词
茄子
果色
上位基因
花青素
遗传规律
eggplant
peel color
epistatic gene
anthocyanin
genetic law
