摘要
为找到表达稳定性最好的内参基因,利用实时荧光定量PCR技术分析了4个内参基因ef1-a、Actin、β-tubulin2和β-tubulin3在野生马铃薯S.acaule冷驯化前后的表达稳定性。结果表明:β-tubulin2和ef1-a表达均稳定,Actin变化最大。因此,β-tubulin2和ef1-a适宜作为野生马铃薯S.acaule的内参基因。
Four reference gens,ef1-a,Actin,β-tubulin2 and β-tubulin3,were systematically compared in wild potato Solanum acaule before and after cold acclimation using Real-time PCR to find out the reference gene with the best expression stability.The results showed that the expression of β-tubulin2 and ef1-a were stable in wild potato S.acaule before and after cold acclimation,the expression of Actin was the most unstable.The result indicated that β-tubulin2 and ef1-a were suitable as reference genes for wild potato S.acaule before and after cold acclimation.
出处
《西南农业学报》
CSCD
北大核心
2012年第5期1592-1595,共4页
Southwest China Journal of Agricultural Sciences
基金
农业部薯类作物生物学与遗传育种重点实验室项目
耐冻野生马铃薯遗传改良研究与应用[黔科合外J字(2011)7032]
