摘要
为探究不同温度条件下添加碳酸钙对活性有机碳组分及微生物群落组成的影响,以贵州典型黄壤土为研究对象,采用同位素标记法,设置6个处理(培养温度分别为15,25,35℃下添加碳酸钙和不添加碳酸钙),通过分析不同温度下碳酸钙添加对土壤活性有机碳、微生物群落组成的影响,揭示外源碳在土壤活性碳库中的分配规律,以期为贵州典型黄壤有机碳固存和改良提供理论依据。结果表明:各温度下添加碳酸钙显著增加土壤DOC和MBC含量(p<0.01),添加碳酸钙各处理土壤DOC含量在培养第5天均达到最大值,相较于不添加碳酸钙处理,添加碳酸钙处理土壤DOC含量在15,25,35℃下分别显著提高83.41%,80.37%,90.41%;添加碳酸钙处理温度对土壤DOC、MBC具有显著影响(p<0.05),在培养的第15,60天,土壤DOC和MBC含量均在不同温度下达到显著性差异,土壤DOC含量大小依次为35,15,25℃,土壤MBC含量多少依次为15,25,35℃。同位素标记发现,在培养第1和第5天,^(13)C-DOC,^(13)C-MBC含量在15,25,35℃下达到峰值,且^(13)C-DOC对土壤总DOC贡献率分别为16.85%,21.20%和15.22%,^(13)C-MBC对土壤总MBC的贡献率分别11.95%,10.49%和17.18%。添加碳酸钙各处理均显著提高土壤pH。高通量测序发现,在培养第60天,外源碳酸钙输入对细菌群落组成相对丰度及群落结构具有显著影响,但对真菌群落组成影响较小。添加碳酸钙处理提高变形菌门相对丰度,降低绿弯菌门相对丰度。相关分析表明,土壤pH与优势菌门变形菌门(Proteobacteria)、担子菌门(Basidiomycota)呈显著正相关,与绿弯菌门(Chloroflexi)、子囊菌门(Ascomycota)呈显著负相关;绿弯菌门与土壤DOC呈显著负相关,放线菌门与土壤MBC、^(13)C-DOC及担子菌门与DOC、^(13)C-MBC、^(13)C-DOC呈显著正相关。综上表明,外源碳酸钙对土壤活性有机碳的影响大于温度,外源碳酸钙增加土壤pH、DOC和MBC含量,改变土壤微生物群落组
In order to explore the effects of adding calcium carbonate on active organic carbon components and microbial community composition under different temperature conditions,the typical yellow soil in Guizhou province was taken as the research object,and six treatments were set up(the treatments with and without adding calcium carbonate at culture temperatures of 15,25,35℃)by isotope labeling method.By analyzing the effects of calcium carbonate addition on soil active organic carbon and microbial community composition at different temperatures,the distribution rule of foreign carbon in soil active carbon pool was revealed,with a view to providing a theoretical basis for organic carbon sequestration and improvement of typical yellow soil in Guizhou.The results showed that:The addition of calcium carbonate significantly increased the contents of DOC and MBC in soil at different temperatures(p<0.01),and the contents of DOC in soil treated with calcium carbonate reached the maximum value on the 5th day of culture.Compared with the treatment without calcium carbonate,the soil DOC content in the treatment with calcium carbonate were significantly increased by 83.41%,80.37%and 90.41%at 15,25 and 35℃,respectively.The temperature of calcium carbonate addition had significant effects on soil DOC and MBC(p<0.05).On the 15th and 60th day of culture,the contents of DOC and MBC in soil showed significant differences at different temperatures,and the overall content of DOC in soil was 35℃>15℃>25℃,and the overall content of MBC was 15℃>25℃>35℃.Isotopic labeling showed that the contents of ^(13)C-DOC and ^(13)C-MBC peaked at 15,25 and 35℃on the 1st and 5th day of culture,and the contribution rate of ^(13)C-DOC to the total soil DOC was 16.85%,21.20%and 15.22%,respectively.The contribution rate of ^(13)C-MBC to total soil MBC was 11.95%,10.49%and 17.18%,respectively.The addition of calcium carbonate significantly increased soil pH.High-throughput sequencing showed that the input of exogenous calcium carbonate had si
作者
赵娜
李瑞东
王小利
段建军
郑钦文
李明瑞
ZHAO Na;LI Ruidong;WANG Xiaoli;DUAN Jianjun;ZHENG Qinwen;LI Mingrui(College of Agriculture,Guizhou University,Guiyang 550025;College of Tobacco,Guizhou University,Guiyang 550025)
出处
《水土保持学报》
CSCD
北大核心
2023年第6期238-245,共8页
Journal of Soil and Water Conservation
基金
国家自然科学基金项目(31860160)。
关键词
外源碳酸钙
温度
活性有机碳组分
微生物群落组成
exogenous calcium carbonate
temperature
active organic carbon component
microbial community composition
