摘要
目的探讨U87-MG细胞中沉默NIN1/RPN12结合蛋白1同源物(NOB1)在宫颈癌及癌前病变中表达及其与高危型人乳头瘤病毒(HPV)DNA负荷量相关性。方法选取2019年1月至2021年3月于本院就诊的120例宫颈病变患者作为研究对象,其中72例宫颈上皮内瘤病患者作为宫颈上皮内瘤病组,48例宫颈癌患者作为宫颈癌组;另选取同期50例体检的宫颈正常女性作为宫颈正常组。3组均进行NOB1检测及高危型HPV检测,比较3组宫颈组织中NOB1基因阳性表达率、高危型HPV DNA负荷量及阳性率,比较宫颈癌患者不同参数的NOB1基因和高危型HPV表达情况,分析NOB1基因阳性表达与高危型HPV DNA负荷量相关性。结果3组NOB1基因阳性表达率比较差异有统计学意义(P<0.05);宫颈癌组NOB1基因阳性表达率高于宫颈癌上皮内瘤病变组、宫颈正常组,宫颈癌上皮内瘤病变组高于宫颈正常组,差异有统计学意义(P<0.05)。3组高危型HPV DNA负荷量比较差异有统计学意义(P<0.05);宫颈癌组高危型HPV DNA阳性率高于宫颈癌上皮内瘤病变组、宫颈正常组,宫颈癌上皮内瘤病变组高于宫颈正常组,差异有统计学意义(P<0.05)。宫颈癌患者组织学分级、淋巴结转移NOB1基因阳性表达率比较差异有统计学意义(P<0.05);宫颈癌患者不同年龄、组织学类型、肌层浸润、分期的NOB1基因阳性表达率比较差异无统计学意义;宫颈癌患者不同年龄、组织学类型、组织学分级、肌层浸润、分期、淋巴结转移的高危型HPV DNA阳性表达率比较差异无统计学意义。Spearman相关性分析结果显示,宫颈癌患者NOB1表达与高危型HPV DNA负荷量呈正相关(r>0,P<0.05)。结论宫颈癌组织中NOB1基因阳性表达,可增加疾病恶性程度和淋巴结转移风险,与高危型HPV感染密切相关。
Objective To investigate the expression of silent NIN1/RPN12 binding protein 1 homolog(NOB1)in U87-MG cells in cervical cancer and and precancerous lesions and its correlation with high-risk human papilloma virus(HPV)DNA load.Methods 120 patients with cervical lesions who were treated in our hospital from January 2019 to March 2021 were selected as the research subjects,including 72 patients with cervical intraepithelial neoplasia as the cervical intraepithelial neoplasia group and 48 patients with cervical cancer as the cervical cancer group;another 50 women with normal cervix who underwent physical examination during the same period were selected as the normal cervical group.NOB1 detection and high-risk HPV detection were performed in the three groups,the positive expression rate of NOB1 gene,the load and positive rate of high-risk HPV DNA in cervical tissues among the three groups were compared,the expression of NOB1 gene and high-risk HPV in different parameters of cervical cancer patients was compared,the correlation between the positive expression of NOB1 gene and the load of high-risk HPV DNA was analyzed.Results There was significant difference in the positive expression rate of NOB1 gene among the three groups(P<0.05);the positive expression rate of NOB1 gene in cervical cancer group was higher than that in the cervical intraepithelial neoplasia group and the normal cervical group,and that in the cervical intraepithelial neoplasia group was higher than that in the normal cervical group,the difference was statistically significant(P<0.05).There was significant difference in high-risk HPV DNA load mong the three groups(P<0.05);the positive rate of high-risk HPV DNA in cervical cancer group was higher than that in cervical intraepithelial neoplasia group and normal cervical group,and that in the cervical intraepithelial neoplasia group was higher than that in the normal cervical group,the differences were statistically significant(P<0.05).There were significant difdoi ferences in the positive expression rat
作者
张义娇
李艳云
陈碧琦
ZHANG Yijiao;LI Yanyun;CHEN Biqi(Department of Obstetrics and Gynecology,Jingzhou Third People's Hospital,Jingzhou,Hubei,434100,China;Department of Obstetrics and Gynecology,Yidu Maternal and Child Health Hospital,Yidu,Hubei,443300,China)
出处
《当代医学》
2023年第15期6-9,共4页
Contemporary Medicine
