摘要
以白肉灵芝为试材,采用单因素与L_(16)(4~5)正交实验的方法,研究了不同温度梯度、琼脂浓度、pH、光照时间及培养皿大小对该菇菌丝生长的影响,以期为其后续开发利用提供参考依据。结果表明:白肉灵芝菌丝在温度范围为15~35℃以及其他可控环境因子的所有处理上均能生长,其中在温度27~29℃、琼脂浓度2.0%~3.0%、酸碱度5.00~5.25、光照时间0 h·d^(-1)的各单因素处理下该菇菌丝萌发快,菌落浓密健壮,生长最快,而氧气对其无显著性影响。在温度25℃、琼脂浓度2.5%、酸碱度5.00、光照时间0 h·d^(-1)、培养皿大小90 mm的可控环境因子最佳组合下,白肉灵芝菌丝生长速度最快,达到(9.88±0.51)mm·d^(-1)。综上所述,白肉灵芝菌丝生长受不同可控环境因素的影响较大,且各因素之间有较大的交互作用。
Taking Ganoderma leucocontextum as the test material,the effects of different temperature gradients,agar concentration,pH,light time and petri dish size on the mycelial growth of Ganoderma leucocontextum were investigated by using the method of one-way and orthogonal experiments with L16(45),in order to provide reference for its subsequent exploitation.The results showed that the mycelium of G.leucocontextum could grow on all treatments with a temperature range of 15-35℃and other controllable environmental factors,among which the mycelium germinated quickly,the colony was dense and robust,and the growth was the fastest under the single-factor treatment of temperature 27-29℃,agar concentration of 2.0%-3.0%,pH 5.00-5.25 and light time of 0 h·d^(-1),while oxygen had no significant effect on it.Under the best combination of controllable environmental factors such as temperature 25℃,agar concentration 2.5%,pH 5.00,light time 0 h·d^(-1),and petri dish size of 90 mm,the mycelial growth rate of G.leucocontextum was the fastest,reaching(9.88±0.51)mm·d^(-1).In summary,the growth of G.leucocontextum was greatly affected by different controllable environmental factors,and there was a greater interaction between various factors.
作者
康晟菀
周启武
刘迪
周会明
杨保云
杨毅昌
KANG Shengwan;ZHOU Qiwu;LIU Di;ZHOU Huiming;YANG Baoyun;YANG Yichang(School of Biotechnology and Engineering,West Yunnan University,Lincang,Yunnan 677000;Agricultural College,Yanbian University,Yanji,Jilin 133002)
出处
《北方园艺》
CAS
北大核心
2023年第23期107-114,共8页
Northern Horticulture
基金
临沧市科技创新人才培养资助项目(202204AC100001-RC01)
中央引导地方科技发展资金资助项目(202207AB110007)。
关键词
白肉灵芝
菌丝生长
可控环境条件
正交实验
Ganoderma leucocontextum
mycelial growth
controlled environmental condition
orthogonal experiment
