摘要
为了研究继代次数对暗褐网柄牛肝菌(Phlebopus portentosus)菌种活性的影响,选取生产上优良性状发生退化的栽培菌株11023为研究对象,监测其及继代2次,5次,8次后菌种的各项生理生化特征以及出菇性状。结果显示,随着继代次数增加,菌丝长势减弱,菌落生长速率降低,菌落颜色变浅,菌落边缘整齐度降低;淀粉酶分泌能力减弱;出菇率和单菇重均减小,抗杂能力减弱。推断菌株11023继代次数不宜超过2代。
Mycelial and fruit body growth characteristics of Phlebopus portentosus strains 11023-2,11023-5and 11023-8（derived from cultivar 11023 after two,five and eight sub-cultures for 28-30 days at 30 ℃,respectively on a medium consisting of 200 g potato,20 g glucose,2.0g yeast extract,1.0g MgSO_4·7H_2O,1.0g KH2PO4,15 g agar and 1000 mL ddH_2O）were determined using cultivars 11023 and 13012as controls.Increasing frequency of sub-culture resulted in decreasing mycelial growth rates and vigor,lighter colored mycelia,more irregular-edged fungal colonies,and smaller fungal colony：colored zone diameter ratios in Congo red and Lugol＇s iodine starch tests,compared with controls.In cultivation tests using artificial logs composed of 10 kg rubber tree（Hevea brasiliensis）sawdust,5kg rice seeds,15.0g MgSO_4,5.0g KH_2PO_4 and 60% water,adjusted to pH 4-6,increasing frequency of sub-culture resulted in lower fruiting rates and single mushroom fruit body weights,and higher contamination rates,compared with controls.
出处
《食用菌学报》
CSCD
北大核心
2016年第2期44-48,共5页
Acta Edulis Fungi
基金
云南省应用基础研究计划项目(2013FZ168)
云南省科研院所技术开发专项(No.2015DC014)
中国科学院"西部之光"人才培养项目(No.326)
国家自然科学基金项目(31560008)
云南省热带作物科技创新体系建设专项(RF2015-5)资助
关键词
暗褐网柄牛肝菌
继代次数
菌丝生长
子实体生长
淀粉酶
Phlebopus portentosus
frequency of sub-culture
mycelial growth
fruit body growth
amylase
