摘要
棉花属于相对耐盐作物,但高盐胁迫同样会造成棉花产量和纤维品质的大幅下降。深入挖掘抗盐基因并解析棉花响应盐胁迫的分子机理,对加快棉花抗盐遗传改良育种进程具有重要意义。本研究从棉花响应盐胁迫的转录组数据中鉴定到一个受盐诱导极显著下调表达的AP2/ERF转录因子GhTINY2,并分析了GhTINY2超表达拟南芥的抗盐表型和各生理指标。结果显示,在盐胁迫下,GhTINY2超表达植株的种子萌发率显著下降;脯氨酸、可溶性糖、叶绿素含量等均显著减少;多个盐胁迫响应基因显著下调表达;因而表现出更为严重的叶片萎蔫枯黄表型。通过分析GhTINY2超表达拟南芥中的RNA-seq数据,发现差异表达基因(DEGs)富集到叶绿素代谢、刺激响应等生物过程中,且DEGs均呈下调表达趋势。此外,在棉花中通过病毒诱导的基因沉默(VIGS)试验沉默GhTINY2后,TRV:GhTINY2植株在盐胁迫下叶绿素和脯氨酸含量显著增加,从而增强了棉花的抗盐性。综上,GhTINY2是棉花中一个负调控盐胁迫抗性的重要基因,未来将有望通过现代基因工程技术利用GhTINY2创制耐盐棉花材料。
Cotton is a relatively salt-tolerant crop,but high salt stress leads to a significant decline in cotton yield and fiber quality.Mining the genes involved in salt-tolerance and illuminating the molecular mechanisms that underlie this resistance is of great importance in cotton breeding programs.Here,we identified an AP2/ERF transcription factor GhTINY2 in the transcriptome database from cotton treated with salt,and the relative expression level of GhTINY2 was reduced by salt.Subsequently,the salt-resistant phenotype and physiological indicators of the GhTINY2-overexpression Arabidopsis were analyzed.The results revealed that the GhTINY2-overexpression Arabidopsis had a significant decrease in seed germination rate,the content of proline,soluble sugar,and chlorophyll under salt stress,leading to more severe leaf wilting compared with WT.RNA-seq data from GhTINY2-transgenic Arabidopsis revealed that differentially expressed genes(DEGs)were enriched in a series of biological processes,including chlorophyll metabolism and response to stimulus,and the relative expression level of these DEGs significantly was down-regulated.Moreover,the silence of GhTINY2 in cotton through Virus-induced gene silencing(VIGS)assay showed that TRV:GhTINY2 had a significant increase in chlorophyll and proline content,leading to improved salt tolerance compare with TRV:00.In conclusion,these findings suggest that GhTINY2 was an important gene in cotton that negatively regulated salt stress resistance,and it was expected to create salt-tolerant cotton materials using GhTINY2 gene by modern genetic engineering technology in the future.
作者
肖胜华
陆妍
李安子
覃耀斌
廖铭静
闭兆福
卓柑锋
朱永红
朱龙付
XIAO Sheng-Hua;LU Yan;LI An-Zi;QIN Yao-Bin;LIAO Ming-Jing;BI Zhao-Fu;ZHUO Gan-Feng;ZHU Yong-Hong;ZHU Long-Fu(State Key Laboratory of Conservation and Utilization of Agro-Biological Resources in Subtropical Region/College of Agriculture,Guangxi University,Nanning 530000,Guangxi,China;State Key Laboratory of Crop Genetic Improvement,Huazhong Agricultural University,Wuhan 430000,Hubei,China)
出处
《作物学报》
CAS
CSCD
北大核心
2024年第1期126-137,共12页
Acta Agronomica Sinica
基金
广西大学高层次人才科研启动基金项目(A3310051044)
广西大学农学院科研发展金项目(EE101711)资助。
