摘要
一心维纳斯蝴蝶兰(PhalaenopsisI-HsinVenus)花型优雅、花期持久、花香馥郁,是优良的香花品种,具有较高的园林观赏价值和经济价值。为筛选适用于一心维纳斯蝴蝶兰花香物质合成相关基因表达分析的内参基因,本研究以一心维纳斯蝴蝶兰不同花期(中蕾期、初开期、盛开前期、盛开中期、盛开后期、衰败期)的转录组数据为基础,选取了ACT1、ACT2、ACT3、GAPDH、EF1α、TUA、TUB和Ubi共8个管家基因作为候选内参基因,利用实时荧光定量PCR(RT-qPCR)技术检测候选内参基因在花朵不同发育时期的表达情况,并利用geNorm、NormFinder、BestKeeper三个内参基因稳定性分析软件对候选内参基因进行分析。综合分析结果表明,ACT1的稳定性最高,可作为一心维纳斯蝴蝶兰相关基因表达分析的最适内参基因。
Phalaenopsis I-Hsin Venus is an excellent variety of fragrant flowers,with elegant flower types,long flowering period and rich floral fragrance,which has high garden ornamental value.ACT1,ACT2,ACT3,GAPDH,EF1α,TUA,TUB and Ubi,were selected as the candidate internal reference genes based on the transcriptome data of different flower development stages in Phal.I-Hsin Venus to select the appropriate reference genes for RT-qPCR analysis of the correlated genes in the biosynthesis pathway of the floral scent in Phalaenopsis I-Hsin Venus.The expression of the candidate internal reference genes in inflorescences at different stages was detected by RT-qPCR.The candidate internal parameter genes were analyzed by combining three internal parameter gene stability analysis software:geNorm,NormFinder and BestKeeper.The results of comprehensive analysis showed that ACT1 was the most stable and could be used as the best internal reference gene for the expression analysis of Phal.I-Hsin Venus.
作者
章杨婷
张燕萍
黄静妍
王文君
童妍
赵凯
周育真
ZHANG Yangting;ZHANG Yanping;HUANG Jingyan;WANG Wenjun;TONG Yan;ZHAO Kai;ZHOU Yuzhen(College of Landscape Architecture and Art,Fujian Agricultural and Forestry University/Key Laboratory of National Forestry and Grassland Administration for Orchid Protection and Utilization,Fuzhou,Fujian 350002,China;College of Life Sciences,Fujian Normal University,Fuzhou,Fujian 350117,China)
出处
《热带作物学报》
CSCD
北大核心
2023年第11期2188-2195,共8页
Chinese Journal of Tropical Crops
基金
福建省自然科学基金项目(No.2023J01283)
福建农林大学优秀硕士论文基金项目(No.1122YS01009)。
