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植物基因表达转录分析中内参基因的选择与应用 被引量:24

Research Progress in Plant Reference Genes
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摘要 管家基因能够在生物体细胞中表达和不断地被转录,对于维持细胞功能发挥重要作用,并在细胞中组成型稳定表达。随着基因表达研究的深入,发现许多管家基因的表达也受到不同程度的调控。选择合适的管家基因作为内参对于准确定量分析目标基因的表达水平至关重要。通过对植物基因表达研究中常用的内参对照基因的表达特性,综述了可以筛选作为内参对照基因的标准和条件的验证。 House-keeping genes,due to their role in basic cellular process,were assumed to express constitutively.Recent study indicated the expression of many housekeeping genes were found to be modified to some extent.Selecting the appropriate house-keeping gene is the premise to precisely quantify gene expression.This paper reviewed the commonly used reference genes in plant research and new progress in validating proper reference genes.
出处 《生物技术通报》 CAS CSCD 北大核心 2011年第7期7-11,共5页 Biotechnology Bulletin
基金 新疆维吾尔自治区科技重大专项(200731138-3) 上海市能源作物育种及应用重点建设实验室开放课题
关键词 植物 内参对照基因 管家基因 基因表达 RT-PCR Plant Reference genes House-keeping gene Gene expression RT-PCR
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参考文献29

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二级参考文献65

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