摘要
为明确引起佛手叶部褐斑病的病原,本研究从重庆市垫江区采集病叶,采用组织分离法分离并纯化得到佛手叶部褐斑病菌菌株CQACMM 22001,依据科赫氏法则验证其致病性。根据病原菌形态学特征及分子生物学鉴定结果,将该菌株鉴定为棉链格孢(Alternaria gossypina)。生物学特性研究表明,该菌对光照不敏感,生长最适培养基为燕麦琼脂培养基,最适生长温度为30℃,最适pH为7.0,最适碳源为可溶性淀粉和乳糖,最适氮源为氯化铵。杀菌剂毒力测定结果表明,咪鲜胺、苯醚甲环唑、异菌脲和啶酰菌胺对该菌有较强的抑制作用,EC50分别为0.008、2.024、4.700和6.294 mg·L^(-1)。本研究为佛手叶部褐斑病的有效防控提供了科学依据。
In order to identify the pathogen causing leaf brown spot on Citrus medica var.sarcodactylis,leaves with typical symptoms of brown spot were collected from Dianjiang District of Chongqing.The fungal strain CQACMM 22001 was isolated by tissue separation and then was purified,and its pathogenicity was verified ac-cording to Koch's postulates.Based on morphological characteristics and the result of molecular identification,the strain was identified as Alternaria gossypina.Research on biological characteristics of the pathogen demon-strated that it is insensitive to light;OA medium is the most suitable medium for its mycelial growth;the optimal temperature for the growth of the pathogen is 30℃,the optimal pH is 7.0,the optimal carbon sources are soluble starch and lactose,and the optimal nitrogen source is ammonium chloride.Toxicity test results showed that prochloraz,difenoconazole,iprodione,and boscalid had stronger inhibitory effect on A.gossypina,with ECso valuesof 0.008,2.024,4.700,and 6.294 mg·L^(-1),respectively.This study provides scientific basis for the prevention and control of leaf brown spot of C.medica var.sarcodactylis.
作者
窦涛
范安阳
崔广林
李隆云
宋旭红
DOU Tao;FAN Anyang;CUI Guanglin;LI Longyun;SONG Xuhong(Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China;Chongqing Sub-center of National Resource,Center for Chinese Materia Medica,China Academy of Chinese Medical Science,Chongqing 400065,China)
出处
《植物病理学报》
CAS
CSCD
北大核心
2023年第5期769-778,共10页
Acta Phytopathologica Sinica
基金
国家中药材产业技术体系(CARS-21)
重庆市科研机构激励引导专项(cstc2020jxjl10003)
重庆市现代山地特色高效农业技术体系(2021[10])。
关键词
佛手
叶片褐斑病
棉链格孢
生物学特性
毒力测定
Citrus medica var.sarcodactylis
leaf brown spot
Alternaria gossypina
biological characteris-tics
toxicity test
