摘要
目的探究丰富环境联合褪黑素对快速老化(SAMP8)小鼠学习记忆功能及DNA氧化损伤的影响。方法 6月龄SPF级健康雄性SAMP8小鼠24只,采用随机数字表法分为模型组、丰富环境组、褪黑素组和丰富环境+褪黑素组,每组6只;6只同源同月龄SAMR1小鼠为对照组。丰富环境组和丰富环境+褪黑素组小鼠饲养于丰富环境,同时褪黑素组、丰富环境+褪黑素组小鼠皮下注射褪黑素8 mg/(kg·d),模型组、对照组、丰富环境组小鼠皮下注射等体积0.9%氯化钠溶液,1次/d,共28 d。采用老化度评分评估小鼠老化情况;Morris水迷宫与Y迷宫实验评估小鼠学习记忆能力;苏木精-伊红染色法观察小鼠海马区细胞形态,免疫组织化学染色法检测小鼠海马区Aβ1-42蛋白水平;Western blot法、酶联免疫吸附法检测小鼠海马区DNA损伤标志蛋白磷酸化组蛋白H2A家庭成员X(γ-H2AX)、8-羟基脱氧鸟苷(8-OHdG)的水平。采用SPSS 25.0统计软件处理数据,多组间比较采用单因素方差分析,进一步两两比较采用LSD-t检验。结果 (1)5组小鼠干预后老化度评分差异有统计学意义(F=126.4,P<0.01)。干预后,丰富环境组、褪黑素组、丰富环境+褪黑素组小鼠老化度评分均低于模型组(均P<0.05),且丰富环境+褪黑素组小鼠显著低于丰富环境组(P<0.05)。(2)5组小鼠逃避潜伏期的时间-组别交互作用、组别主效应、时间主效应均显著(F=11.2,799.9,121.8,均P<0.01)。第2~4天,丰富环境组、褪黑素组、丰富环境+褪黑素组小鼠逃避潜伏期均显著低于模型组(P<0.05)。5组小鼠目标象限停留时间与跨平台次数均差异具有统计学意义(F=70.38,48.83,均P<0.01)。丰富环境组、褪黑素组、丰富环境+褪黑素组小鼠目标象限停留时间与跨平台次数显著高于模型组(均P<0.05)。(3)5组小鼠总交替次数及正确交替率均差异具有统计学意义(F=291.328,113.482,均P<0.01)。褪黑素组和丰富环境+褪黑素组小鼠
Objective To explore the effects of enriched environment combined with melatonin on learning and memory function and DNA oxidative damage in senescence accelerated mouse prone 8(SAMP8)mice.Methods Twenty-four 6-month-old SPF healthy male SAMP8 mice were randomly divided into model group,enriched environment group,melatonin group and enriched environment+melatonin group,with 6 mice in each group.Six homologous SAMR1 mice of the same age were used as the control group.The mice in the enriched environment group and the enriched environment+melatonin group were fed in the enriched environment.At the same time,the mice in the melatonin group and the enriched environment+melatonin group were subcutaneously injected with melatonin(8 mg/(kg·d))once a day for 28 d.The mice in the model group,the control group and the enriched environment group were subcutaneously injected with an equal volume of 0.9%sodium chloride solution once a day for 28 days.Aging score was used to evaluate the aging of mice.Morris water maze and Y maze tests were used to evaluate the learning and memory ability of mice.The cell morphology of hippocampus in mice was observed by hematoxylin-eosin staining,and the level of Aβ1-42 protein in hippocampus of mice was detected by immunohistochemical staining.The levels ofγ-H2A histone family member X(γ-H2AX)and 8-hydroxy-2 deoxyguanosine(8-OHdG)proteins in hippocampus of mice were detected by Western blot and Enzyme-linked immunosorbent assay.SPSS 25.0 statistical software was used to process the data.One-way analysis of variance was used for comparison among multiple groups,and LSD-t test was used for further pairwise comparison.Results(1)There was a statistical difference in aging scores among the 5 groups of mice after intervention(F=126.4,P<0.01).After intervention,the aging scores of mice in the enriched environment group,melatonin group,and enriched environment+melatonin group were lower than that in the model group(all P<0.05),and the score of the enriched environment+melatonin group was signifi
作者
屈海峰
杜鹃
李建民
王一超
王继成
罗光新
田子玉
刘俊杰
Qu Haifeng;Du Juan;Li Jianmin;Wang Yichao;Wang Jicheng;Luo Guangxin;Tian Ziyu;Liu Junjie(Clinical Medical College,North China University of Science and Technology,Tangshan 063000,China;School of Basic Medical Sciences,North China University of Science and Technology,Tangshan 063000,China;The Affilated Hospital,North China University of Science and Technology,Tangshan 063000,China;School of Public Health,North China University of Science and Technology,Tangshan 063000,China)
出处
《中华行为医学与脑科学杂志》
CAS
CSCD
北大核心
2023年第11期968-975,共8页
Chinese Journal of Behavioral Medicine and Brain Science
基金
河北省2019年政府资助临床医学优秀人才培养和基础课题研究项目
唐山市科技计划项目(21130224C)
河北省2022年度医学科学研究课题计划(20221533)
华北理工大学"大学生创新创业训练计划项目"(X2022297)。
