摘要
目的探讨美金刚联合丰富环境对快速衰老小鼠（SAMP8）海马脑源性神经营养因子（BDNF）、酪氨酸蛋白激酶受体B（TrkB）表达及学习记忆能力的影响。方法24只雄性SAMP8小鼠随机分为对照组、丰富环境治疗组（丰富环境组）、美金刚治疗组（美金刚组）和美金刚联合丰富环境组（联合治疗组），每组6只小鼠。治疗8周后，采用Morris水迷宫试验检测小鼠空间学习记忆能力，免疫印记法和实时定量PCR法检测小鼠海马组织中BDNF、TrkB的表达水平。结果与对照组比较，丰富环境组、美金刚组小鼠在试验第3d起寻找到平台的潜伏期明显缩短，联合治疗组小鼠在试验第2d起的逃避潜伏期明显缩短（P〈0．05～0．01）；且联合治疗组小鼠在试验第4d时的逃避潜伏期明显短于丰富环境组和美金刚组（均P〈0．05）。与对照组比较，其余3组小鼠穿越平台次数明显增多（P〈0．05～0．01）；且联合治疗组小鼠穿越平台次数明显多于丰富环境组和美金刚组（均P〈0．01）。与对照组比较，其余3组小鼠海马BDNFmRNA、BDNF蛋白和TrkB蛋白的表达均明显升高（P〈0．05～0．01），其中联合治疗组明显高于丰富环境组与美金刚组（P〈0．05～0．01）。各组小鼠海马TrkBmRNA表达的差异无统计学意义。结论美金刚和丰富环境治疗能够有效地改善SAMP8小鼠学习记忆能力并增加海马BDNF和TrkB的表达，二者联合治疗时效果更显著。
Objective To explore the effects of Memantine combined with enriched environment （EE） on the expression of brain derived neurophic factor （BDNF） and tyrosine kinase receptor B （TrkB） in hippocampus and the learning, memory ability of senescence accelerated mouse prone 8 （SAMP8）. Methods Twenty-four male SAMP8 mouse were randomly divided into control group, EE group, Memantine group and Mematine combined with EE group （ME group）, 6 mouse in each group. After 8-week-treatment, Morris water maze test was performed to evaluate improvement of spatial learning, memory ability. The expression of BDNF and TrkB was detected by Western blot and Real time PCR. Results Compared with SE group, the overall mean escape latency of EE group and Memantine group was significantly shorten since the third day, and which of EM group was shorten since the second day （ P 〈 0. 05 -0. 01 ） ; and the mean escape latency of EM group was shorter than EE and Memantine groups in the fourth day （all P 〈 0. 05 ）. Compared with control group, the frequencies of crossing platform in the other 3 groups were significantly increased （ P 〈 0. 05 - 0. 01 ） ; and the frequencies of crossing platform in the ME group was more than EE and Memantine groups （ all P 〈 0. O1 ）. Compared with control group, the levels of BDNF mRNA, protein and TrkB protein in the other 3 groups were significantly increased （ P 〈 0. 05 - 0. 01 ） ; and which in ME group were significantly higher than the EE and Memantine groups （P 〈 0.05 -0.01 ）. However,the difference of levels of TrkB mRNA between each group was no statistical significance. Conclusions The Memantine and EE can improve the learning, memory ability of SAMP8 mouse, and increase the expression of the BDNF and TrkB in hippocampus. The effects can be more significant when Memantine combined with EE.
出处
《临床神经病学杂志》
CAS
北大核心
2013年第4期278-281,共4页
Journal of Clinical Neurology
基金
江苏省自然科学基金(BK2008081)
南京医科大学科技发展基金(2010NJMUZ44)
关键词
快速衰老小鼠
美金刚
丰富环境
脑源性神经营养因子
酪氨酸蛋白激酶受体
B
senescence accelerated mouse prone 8
Memantine
enriched environment
brain derivedneurophie factor
tyrosine kinase receptor B
