摘要
同轴静电纺丝技术是基于传统静电纺丝技术改进,其制备的纤维材料不仅具有高比表面积,还具有特殊的核壳结构,能将活性分子等包覆在核内,保持其生物活性,达到缓释目的。本文通过同轴静电纺丝技术,制备具有核壳结构的明胶(Gelatin,GEL)@左旋聚乳酸(Poly(L-lactic acid),PLLA)纳米纤维膜。利用扫描电子显微镜(SEM)、激光共聚焦显微镜(LSCM)、拉伸测试及接触角测试等对纳米纤维膜的形貌结构和性能进行表征。探讨了静电纺丝工艺参数对纳米纤维形貌的影响,并考察了纳米纤维膜的生物相容性。结果表明:所制备的GEL@PLLA核壳纳米纤维表面光滑且有明显的核壳结构,增大核层与壳层流速比,纳米纤维的平均直径从231.41 nm增大到279.49 nm;增加纺丝电压,纤维直径逐渐减小;增加接收距离,纤维直径先减小后增大;核壳结构的GEL@PLLA纤维膜接触角为126.7°,与纯GEL纤维膜相比,表现为疏水性;力学测试结果显示GEL@PLLA核壳纳米纤维具有较好的柔性和弹性;体外细胞培养结果显示,第4代骨髓间充质干细胞(BMSCs)能在GEL@PLLA核壳纤维膜上黏附和增殖,表明该核壳纳米纤维膜具有较好的生物相容性。本文可为纤维膜进一步应用于药物控释系统及生物医用领域奠定基础。
Coaxial electrospinning technology is based on the improvement of traditional electrospinning technology.The fiber material prepared by it not only has a high specific surface area,but also has a special core-shell structure,which can encapsulate active molecules,maintain their biological activity,and achieve the goal of sustained release.This article uses coaxial electrospinning technology to prepare gelatin(GEL)@poly(L-lactic acid)(PLLA)nanofiber membranes with core-shell structure.The morphology,structure,and properties of nanofiber membranes were characterized using scanning electron microscopy(SEM),laser confocal microscopy(LSCM),tensile testing,and contact angle testing.The influence of electrospinning process parameters on the morphology of nanofibers was explored,and the biocompatibility of nanofiber membranes was investigated.The results showed that the surface of the prepared GEL@PLLA core-shell nanofibers was smooth and had a clear core-shell structure.Increasing the flow velocity ratio between the core-shell layers increases the average diameter of the nanofibers from 231.41 nm to 279.49 nm.Increasing the spinning voltage gradually reduces the fiber diameter.By increasing the receiving distance,the fiber diameter decreases first and then increases.The contact angle of the GEL@PLLA fiber membrane is 126.7°,which exhibits hydrophobicity compared to pure GEL fiber membrane.The mechanical test results show that GEL@PLLA core-shell nanofibers have good flexibility and elasticity.The results of in vitro cell culture showed that bone marrow mesenchymal stem cells(BMSCs)can adhere and proliferate on the GEL@PLLA fiber membrane,indicate that the core-shell nanofiber membrane has good biocompatibility.This study can lay the foundation for the further application of fiber membranes in drug-controlled release systems and biomedical fields.
作者
成锡婷
辜鹏程
胡辉
董宇涵
姜强
范佳
杨旋
白燕
CHENG Xiting;GU Pengcheng;HU Hui;DONG Yuhan;JIANG Qiang;FAN Jia;YANG Xuan;BAI Yan(School of Pharmacy,Chongqing Medical University,Chongqing 400016,China;Experimental Teaching Center,Chongqing Medical University,Chongqing 400016,China)
出处
《复合材料学报》
EI
CAS
CSCD
北大核心
2023年第11期6374-6382,共9页
Acta Materiae Compositae Sinica
基金
重庆市自然科学基金面上项目(cstc2017jcyjAX0029)
重庆医科大学青年创新团队发展支持计划(W0055)
重庆医科大学大学生科学研究与创新实验项目(SIEP202194,202229)
重庆市研究生科研创新项目(CYS22370)。
关键词
明胶
左旋聚乳酸
同轴静电纺丝
核壳结构
纳米纤维
药物缓释
gelatin
poly(L-lactic acid)
coaxial electrospinning
core shell structure
nanofiber
drug-controlled release
