摘要
为获得优良的解钾菌(KSB)并明确其对植物产量和品质的影响,采用硅酸盐细菌培养基从紫花苜蓿根际土壤分离、筛选到2株具有高效解钾能力的菌株。通过细菌形态学、16S rDNA序列分析和生理生化鉴定,确定2株解钾菌分别为巨大芽孢杆菌和耐寒短杆菌,命名为XLT-4和XLT-7。2株菌株均具有产吲哚-3-乙酸(IAA)、铁载体和溶磷能力。接种XLT-4和XLT-7显著提高了紫花苜蓿的株高、根长、地上和地下干鲜重,以及根系活力、叶片的磷、钾和粗蛋白含量,降低了中性洗涤纤维和酸性洗涤纤维的含量。此外,接种2株解钾菌还提高了紫花苜蓿根际土壤酶活性和速效钾含量。本研究获得的2株解钾菌在提高紫花苜蓿产量和品质方面发挥了重要作用,是开发微生物制剂的优质菌株资源。
The study aimed to isolate and screen high-efficiency potassium solubilizing bacteria(KSB),and to quantify the effects of KSB application on plant yield and quality.The KSB strains were screened by silicate bacteria media from the rhizosphere soil of alfalfa(Medicago sativa),and subsequently the strains were identified by morphological,16S rDNA sequence,and physiological and biochemical analysis.Pot experiments were conducted to evaluate the effect of inoculation with KSB strains on the yield and quality of alfalfa.Two high-efficiency KSB strains XLT-4 and XLT-7 were obtained with potassium solubilization rates of 10.53%and 9.75%>CK,and identified as Priestia megaterium and Peribacillus frigoritolerans,respectively.These two KSB strains had the metabolic capacity for phosphorus solubilization,siderophore-production and indole acetic acid secretion.Inoculation with XLT-4 and XLT-7 significantly increased plant height,root length,dry and fresh weight above ground and below ground,root activity,the contents of P,K and crude protein in leaves,while the contents of neutral detergent fiber and acid detergent fiber were significantly lower than in CK plants.In addition,the inoculation of the two KSB also improved the enzyme activity and available potassium content in alfalfa rhizosphere soil.In this study,the two KSB strains made a major contribution to improving the yield and quality of alfalfa,and these two organisms are high quality strain resources for the development of microbial preparations.
作者
王彦佳
胡伯昂
陈佳欣
许丽婷
姚琳
冯丽荣
郭长虹
WANG Yan-jia;HU Bo-ang;CHEN Jia-xin;XU Li-ting;YAO Lin;FENG Li-rong;GUO Chang-hong(Heilongjiang Provincial Key Laboratory of Molecular Cellular Genetics and Genetic Breeding,College of Life Science and Technology,Harbin Normal University,Harbin 150000,China;Heilongjiang Guohong Energy Conservation and Environmental Protection Co.,LTD.,Harbin 150028,China)
出处
《草业学报》
CSCD
北大核心
2023年第12期139-149,共11页
Acta Prataculturae Sinica
基金
国家自然科学基金(U21A20182,31972507)
黑龙江省科技攻关项目(2021ZXJ03B05)
哈尔滨师范大学研究生创新基金(HSDSSC X2022-38)资助。
关键词
紫花苜蓿
解钾菌
巨大芽孢杆菌
耐寒短杆菌
产量
品质
alfalfa
potassium-solubilizing bacteria
Priestia megaterium
Peribacillus frigoritolerans
herbage yield
herbage nutritive quality
