摘要
为获得高效降解T-2毒素的微生物菌株并探究其降解机制,本研究以T-2毒素为底物从小麦样品中筛选降解微生物并进行鉴定,探究该菌株对T-2毒素的降解特性,利用超高效液相色谱-飞行时间质谱仪分析其代谢产物与降解机制。结果表明,从50份小麦样品中筛选到2株T-2毒素高效降解菌株AFJ-2和AFJ-3,通过形态学观察和16S rDNA序列分析,初步鉴定为短小杆菌属(Curtobacterium sp.)菌株和芽孢杆菌属(Bacillus sp.)菌株。菌株AFJ-2和AFJ-3分别能在7 h和12 h内将5μg/mL T-2毒素完全降解,2株菌的胞内酶均对T-2毒素具有显著降解效果(P<0.05),不存在吸附作用。菌株AFJ-2可将T-2毒素转化为HT-2毒素和T-2三醇,菌株AFJ-3降解T-2毒素产生新茄镰孢菌醇(neosolaniol,NEO),基于降解位点推测,2株菌共同作用于T-2毒素可产生新的产物4-脱乙酰-NEO。研究结果丰富了生物降解T-2毒素的菌种资源库,为T-2毒素降解酶的分离纯化及功能基因的挖掘提供一定参考依据。
The purpose of this study was to obtain microbial strains capable of high-efficiency degradation of T-2 toxin and to investigate their mechanism of action.T-2 toxin-degrading microorganisms were isolated from wheat and identified and their degradation characteristics were explored;their metabolites and mechanism of action were analyzed by ultra-high performance liquid chromatography-quadrupole time-of-flight mass spectrometry(UPLC-Q-TOF-MS).The results showed that two efficient T-2 toxin degrading strains,AFJ-2 and AFJ-3,were screened from 50 wheat samples and identified respectively as Curtobacterium sp.and Bacillus sp.by morphological observation and 16S rDNA sequence analysis.T-2 toxin at 5μg/mL was completely degraded within 7 and 12 h by strains AFJ-2 and AFJ-3,respectively,and intracellular enzymes from them could significantly degrade T-2 toxin(P<0.05)without adsorption.Strain AFJ-2 was able to convert T-2 toxin to HT-2 toxin and T-2 triol,while strain AFJ-3 could degrade T-2 toxin to produce neosolaniol(NEO).Based on the predicted degradation sites,it was hypothesized that the two strains could work together to degrade T-2 toxin to produce a new product,4-deacetyl-NEO.The research results enrich the strain resource bank for the biodegradation of T-2 toxin,and provide a reference for the separation and purification of T-2 toxin-degrading enzymes and the excavation of functional genes.
作者
马妍
孙长坡
王峻
杜稳
刘虎军
周文化
赵一凡
MA Yan;SUN Changpo;WANG Jun;DU Wen;LIU Hujun;ZHOU Wenhua;ZHAO Yifan(College of Food Science and Engineering,Central South University of Forestry and Technology,Changsha 410004,China;Academy of National Food and Strategic Reserves Administration,Beijing 100037,China)
出处
《食品科学》
EI
CAS
CSCD
北大核心
2023年第22期173-182,共10页
Food Science
基金
中央级公益性科研院所基本科研业务费专项(JY2209
ZX2236)。
关键词
T-2毒素
筛选
鉴定
生物降解
代谢产物
降解机制
T-2 toxin
screening
identification
biodegradation
metabolites
degradation mechanism
