摘要
设计一种紫外检测器(UVD)与荧光检测器(FLD)串联的高效液相色谱法(HPLC)测定维生素B2片含量的方法,并应用于实验教学。该实验方案能克服常规HPLC法(单一检测器)存在的缺限,同时实验过程中学生不仅掌握了HPLC的操作,而且能了解UVD和FLD的原理及应用,利于培养学生的综合检测能力和拓展思维能力。
The high performance liquid chromatography(HPLC)method of UV detector-fluorescence detector(UVD-FLD)connected in series for the determination of vitamin B2 in tablets was designed as a teaching experiment.This method can overcome the limitations of common HPLC by a single detector.Through the application of this method for experimental teaching,students could not only master the basic theory and operation of HPLC,but also understand the principles and functions of UVD and FLD.Furthermore,it also improved the undergraduate students’experimental skill and comprehension.
作者
谢一凡
姚莉韵
杨若林
蔡玉兴
金玉杰
李宁
Yifan Xie;Liyun Yao;Ruolin Yang;Yuxing Cai;Yujie Jin;Ning Li(Basic Medical Experimental Teaching Center,School of Medicine,Shanghai Jiao Tong University,Shanghai 200025,China;Medical Chemistry and Bioinformatics Center,School of Medicine,Shanghai Jiao Tong University,Shanghai 200025,China)
出处
《大学化学》
CAS
2023年第9期196-201,共6页
University Chemistry
基金
上海交通大学医学院课程建设基金项目(BJ13000180095)。
