摘要
目的:建立薄层色谱与表面增强共振拉曼光谱联用技术检测苯乙胺类拟肾上腺素硫酸沙丁胺醇药物残留的方法。方法:使用微波加热法用硝酸银和柠檬酸钠制备表面增强剂银溶胶;对所制备的银溶胶进行参数测定,选择表面增强效果较好的银溶胶;应用表面增强共振拉曼光谱法检测硫酸沙丁胺醇时,在TLC板分离出的目标斑点上滴加芳香族重氮盐和表面增强剂银溶胶后再检测其拉曼光谱,并对市售猪肝进行样品检测。结果:该方法可显著增强拉曼光谱的检测信号,增强因子可达到1.65×107。结论:本方法专属性强、耐用性好,为苯乙胺类拟肾上腺素药物残留物的鉴别技术提供了一种快速、高效的新方法。
Objective:To establish a method for the detection of phenethylamine-like adrenaline-like salbutamol sulfate residues by TLC and surface enhanced resonance Raman spectroscopy.Method:The surface strengthening agent silver sol was prepared from silver nitrate and sodium citrate by microwave heating method.The parameters of the prepared silver sol were determined,and the silver sol with better surface enhancement effect was selected.When salbutamol sulfate was detected by surface-to-enhanced resonance Raman spectroscopy,aromatic diazo salt and surface enhancer silver sol were added to the target spots isolated from TLC plate,and then the Raman spectra were detected.Result:This method can significantly enhance the detection signal of Raman spectrum,and the enhancement factor can reach 1.65×107.Conclusion:This method has strong specificity and good durability,and provides a rapid and efficient new method for the identification of phenethylamines and adrenergic drug residues.
作者
曹萌
许凤
张宏莲
梁鑫
徐涛
李骞
CAO Meng;XU Feng;ZHANG Honglian;LIANG Xin;XU Tao;LI Qian(School of Pharmacy,Qiqihar Medical University,Qiqihar 161006,China)
出处
《食品安全导刊》
2023年第31期78-82,共5页
China Food Safety Magazine
基金
2022年齐齐哈尔医学科学院项目资助(QMSI2022Z-03)。
关键词
薄层色谱
表面增强共振拉曼光谱法
兽药残留
thin layer chromatography
surface enhanced resonance Raman spectroscopy
veterinary drug residue
