摘要
活性酶普遍存在于各种生命活动中,一些疾病与活性酶的异常表达息息相关,精确检测酶的表达水平以及原位成像,为相关疾病的诊断与治疗提供了有力的判断依据。至今,大量的检测技术已经开发出来,其中以分子荧光探针为代表的光学技术具有非侵袭性以及灵敏度高、检测限低、响应时间快和生物相容性好等优势,在检测活性酶上备受青睐。然而,在使用分子荧光探针检测时,由于小分子容易在酶活性位点发生扩散,无法定位,导致探针时空分辨率较差。因此,为提高成像检测的时空分辨率、降低背景干扰和假阳性,原位成像的设计理念随之提出,已成为生物光学成像的研究热点之一。目前,研究者已报道多种分子荧光探针用于酶的原位成像的设计并取得显著效果。本文将深入介绍用于活性酶检测的分子荧光探针的设计策略及其在原位成像中的研究进展,希望为该领域的研究者们提供一些启发。
Enzyme widely exists in various life activities.Some diseases are closely related to abnormal expression of active enzymes.Accurate detection of enzyme expression levels and in situ imaging provide a powerful basis for di⁃agnosis and treatment of related diseases.Up to now,a large number of detection technologies have been developed,among which the fluorescent technology represented by small-molecule fluorescent probes has advantages such as non-invasive,high sensitivity,low detection limit,fast response time and good biocompatibility.It is favored in the detection of biological enzyme.However,as small-molecule fluorescent probes are used for detection,they tend to diffuse at the active sites of enzyme,resulting in poor spatial and temporal resolution of the probes.Therefore,in or⁃der to improve the spatial and temporal resolution of imaging detection and reduce background interference and false positives,the design of in situ imaging has been proposed,which has become one of the research focuses of optical imaging.At present,researchers have reported that a variety of small-molecular fluorescent probes have been used in the design of enzyme in situ imaging and achieved remarkable results.This review will introduce the design strategy of small-molecular fluorescent probes for enzyme detection and the research progress in situ imaging,hoping to pro⁃vide some inspirations for researchers in this field.
作者
张龙
黄众熙
沈倩
鞠霖杰
吴琼
余昌敏
ZHANG Long;HUANG Zhongxi;SHEN Qian;JU Linjie;WU Qiong;YU Changmin(School of Flexible Electronics(Future Technologies),Nanjing Tech University,Nanjing 211816,China;The Affiliated Cancer Hospital of Nanjing Medical University,Nanjing 210009,China)
出处
《发光学报》
EI
CAS
CSCD
北大核心
2023年第11期2057-2075,共19页
Chinese Journal of Luminescence
基金
国家重点研发计划项目(2020YFA0709900)
国家卫生健康委核医学重点实验室、江苏省分子核医学重点实验室(KF202203)。
关键词
原位成像
分子荧光探针
活性酶
研究进展
in situ imaging
small-molecule fluorescent probes
enzyme
recent progress
